Microbiology Testing FAQs — Methods & Media | HiMedia USA

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What is Bile Esculin Agar used for?

Bile Esculin Agar tests esculin hydrolysis in presence of bile - characteristic of Group D Streptococcus and Enterococcus. Contains: (1) Bile (4%) - inhibits most bacteria, (2) Esculin - hydrolyzed by Group D Strep/Enterococcus, (3) Ferric citrate - reacts with esculetin (esculin breakdown product) producing black color. Positive: growth + black (or brown halo). Used for: (1) Enterococcus identification, (2) Probiotic culture verification (E. faecium common probiotic), (3) Group D Strep testing. Inoculate slant or stab, incubate 48h at 35°C. For differential isolation and presumptive identification of group D Streptococci in food and other products.
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What is MRS Broth used for?

MRS (deMan, Rogosa, and Sharpe) Broth is the standard medium for cultivation and enumeration of Lactobacillus and other lactic acid bacteria. Rich formulation with peptones, beef extract, yeast extract, glucose, and Tween 80. Lower pH (6.2-6.6) favors lactobacilli. Used for: (1) Probiotic product enumeration (CFU/capsule), (2) Fermented food analysis (yogurt, kimchi, sauerkraut), (3) Beer/wine microbiology, (4) Lactobacillus stock cultures. MRS Agar (with agar added) for plate counts. Incubate anaerobically at 35-37°C for 48-72 hours.
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What is Rogosa SL Agar used for?

Rogosa SL Agar is a selective medium for isolation and enumeration of Lactobacillus species from clinical, food, dairy, and probiotic products. Contains: (1) Low pH (5.4) - inhibits most bacteria except Lactobacillus, (2) Sodium acetate - selective agent, (3) High nutrient content. Lactobacillus colonies appear as small white/cream colonies. Used for: (1) Yogurt and fermented food QC, (2) Probiotic product testing, (3) Oral/vaginal lactobacilli in clinical samples, (4) Beer/wine spoilage detection. Incubate anaerobically or in CO2 at 35-37°C for 48-72 hours.
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What is M17 Medium used for?

M17 Medium is selective for thermophilic Lactococcus and Streptococcus species used in dairy fermentation. Developed for Streptococcus thermophilus in yogurt. Contains: (1) Disodium glycerophosphate - buffer, (2) Ascorbic acid - reducing agent, (3) Magnesium sulfate - stimulates lactic acid bacteria. Used for: (1) Yogurt starter culture enumeration, (2) Cheese culture testing, (3) S. thermophilus counts in dairy, (4) Probiotic product QC. Incubate anaerobically at 42-43°C for 48 hours (S. thermophilus optimal). Add agar for solid medium. For cultivation of lactic Streptococci and plaque assay of lactic bacteriophages.
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