Microbiology Testing FAQs — Methods & Media | HiMedia USA

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What is SMAC Agar and why is sorbitol used?

Sorbitol MacConkey Agar (SMAC) is used specifically for E. coli O157:H7 detection. Uses sorbitol instead of lactose. E. coli O157:H7 CANNOT ferment sorbitol → produces colorless colonies. Other E. coli CAN ferment sorbitol → produce pink colonies. This differentiates pathogenic O157:H7 from commensal E. coli. Used in: (1) Ground beef testing, (2) Produce testing, (3) Outbreak investigations, (4) Diarrheal stool samples. Colorless colonies are presumptive O157:H7 requiring confirmation with O157 antigen test and molecular methods (Shiga toxin genes stx1/stx2). The composition and performance criteria are in accordance with ISO 16654:2001&/Amd 1:2017.
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