Microbiology Testing FAQs — Methods & Media | HiMedia USA

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Nutrient Agar
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What is Nutrient Agar?

Nutrient Agar is a general-purpose, non-selective medium for cultivating, isolating and enumerating non-fastidious microorganisms. Because it contains no selective or differential agents, it supports unrestricted growth of most aerobic and facultative anaerobic bacteria. It is used for purity testing, bioburden determination, culture maintenance, environmental monitoring and water analysis, and allows observation of colony morphology, pigmentation and texture for preliminary identification.

What are the composition and formula of Nutrient Agar?

HiMedia Nutrient Agar (M001) contains peptone, sodium chloride, HM Peptone B, yeast extract, and agar, providing essential nutrients and a solidifying matrix for microbial growth. The formulation per litre consists of peptone 5.0 g, sodium chloride 5.0 g, HM Peptone B 1.5 g, yeast extract 1.5 g, and agar 15.0 g. HM Peptone B is equivalent to beef extract. The formula is adjusted and standardized to meet specified performance parameters, with a final pH of 7.4 ± 0.2 at 25°C.

What are the technical specifications of Nutrient Agar?

Nutrient Agar (M001): Nutrient Agar is a general-purpose, non-selective medium for cultivation of less fastidious microorganisms. It contains peptone (5.0 g/L), sodium chloride (5.0 g/L), HM peptone B (1.5 g/L), yeast extract (1.5 g/L), and agar (15.0 g/L). The final pH is 7.4 ± 0.2 at 25°C. The medium forms a light-yellow, clear to slightly opalescent gel with firm gelling comparable to 1.5% agar. Cultural performance is evaluated at 35–37°C for 18–48 hours, with good-to-luxuriant growth and ≥70% recovery for specified test organisms.

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