Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Alongside the ubiquitous Escherichia coli, Saccharomyces cerevisiae (budding yeast) and Schizosaccharomyces pombe (fission yeast) hold immense significance in the realm of molecular biology as crucial model organisms. These seemingly simple single-celled fungi, with their readily manipulatable genomes, serve as invaluable tools for researchers. For growth and development of Saccharomyces and its species HiGenoMB has its own unique enriched Growth Media.
Rich Media
- Yeast Extract-Peptone-Dextrose (YPD) broth/agar: The workhorse for both yeasts, providing all essential nutrients for rapid growth and plasmid maintenance. Contains yeast extract, peptone, and dextrose (glucose).
- Complete Synthetic Medium (CSM): A defined minimal medium supplemented with essential amino acids and vitamins, allowing precise control over nutrient composition and useful for studying specific metabolic pathways or gene expression.
Minimal Media
- Yeast Nitrogen Base (YNB): A base medium lacking a carbon source, allowing customization by adding specific sugars or other carbon sources to study specific metabolic processes. Can be supplemented with various amino acids and vitamins for additional control.
- Edinburgh Minimal Medium (EMM): A minimal medium specifically designed for Schizosaccharomyces pombe, containing essential salts, trace elements, and a carbon source like glucose. Useful for studying Schizosaccharomyces pombe metabolism and cell cycle control.
Selective Media
- Synthetic Defined Media: Synthetically Defined media known as Yeast Nitrogen Base Media for the growth of yeast cells is a synthetic defined media for the selective growth of Saccharomyces cerevisiae for its prototrophic or auxotrophic characteristics.
