Microbiology Testing FAQs — Methods & Media | HiMedia USA

FAQ's Category:
Anaerobic
All Categories Fluid Thioglycollate Medium-FTM HiFill™ Test HiVeg® Medium MacConkey Agar Nutrient Agar Soyabean Casein Digest Medium USP <71> Sterility Testing Media USP <62> Tests for Specified Microorganisms Chromogenic Plates Cannabis Specialty Food Food Safety Environmental Dairy Water Water Pharma Water Testing Additive Anaerobic AST Cannabis Food Chromogenic Media Explained Clinical Fungal Clinical Mycology Clinical Mycobacteria Animal Feeding Stuff Selective Media QC Probiotic Pharmaceutical Mycology Molecular Biology Identification General

What is Thioglycollate Medium USP used for?

Thioglycollate Medium USP is a liquid medium supporting growth of aerobic, anaerobic, and microaerophilic bacteria. Not just for sterility testing - also used for: (1) Cultivation of anaerobes without anaerobic chamber, (2) Determination of oxygen requirements of bacteria, (3) Maintenance of stock cultures, (4) Starting cultures for anaerobic studies, (5) Clostridium enrichment. Contains thioglycollate and cystine as reducing agents creating anaerobic environment in lower tube portions. Resazurin indicator shows oxygen presence (pink) or absence (colorless). Heat to drive off oxygen before use if >1/3 is pink.
Related Products:

What is RCM used for?

Reinforced Clostridial Medium (RCM) is used for cultivation of Clostridium species and other anaerobes. Contains: (1) Peptones and beef extract - rich nutrients, (2) Yeast extract - growth factors, (3) Glucose - energy, (4) Sodium acetate and cysteine - reduce oxygen, (5) Starch and agar - O2 removal. Used for: (1) Clostridium perfringens enumeration in food, (2) C. difficile cultivation, (3) General anaerobe cultivation, (4) Spore studies. Can be used as broth or solidified with extra agar. Incubate anaerobically at 35-37°C.
Related Products:

What is Cooked Meat Medium used for?

Cooked Meat Medium is enriched liquid medium for cultivation of anaerobic bacteria, particularly Clostridium species. Contains: (1) Cooked meat particles - provide reducing environment and nutrients, (2) Peptone, yeast extract - growth factors, (3) Glucose - energy. The meat particles: (a) Create anaerobic conditions, (b) Provide hemin and vitamins, (c) Absorb toxic metabolites. Used for: (1) Clostridium isolation (C. perfringens, C. botulinum), (2) Anaerobe culture, (3) Sterility testing of biologicals (historical), (4) Maintaining anaerobic stock cultures. Turbidity or meat digestion indicates growth.
Related Products:

What is RCM in plate form?

RCM Agar (solidified RCM broth) for anaerobe plate counts, particularly C. perfringens enumeration in food. Incubate anaerobically 35-37°C for 24-48h. Recommended for the cultivation and enumeration of Clostridia and other anaerobes.
Related Products:
  • SKU: MP154

Why use Thioglycollate without indicator?

Some applications require thioglycollate without resazurin indicator (which can inhibit some organisms). Used when resazurin might interfere with testing or organism detection. Recommended for sterility testing of turbid or viscous biological products and for cultivation of anaerobes and microaerophiles from clinical and nonclinical specimens.
Related Products: