Microbiology Testing FAQs — Methods & Media | HiMedia USA

FAQ's Category:
Clinical Mycobacteria
All Categories Fluid Thioglycollate Medium-FTM HiFill™ Test HiVeg® Medium MacConkey Agar Nutrient Agar Soyabean Casein Digest Medium USP <71> Sterility Testing Media USP <62> Tests for Specified Microorganisms Chromogenic Plates Cannabis Specialty Food Food Safety Environmental Dairy Water Water Pharma Water Testing Additive Anaerobic AST Cannabis Food Chromogenic Media Explained Clinical Fungal Clinical Mycology Clinical Mycobacteria Animal Feeding Stuff Selective Media QC Probiotic Pharmaceutical Mycology Molecular Biology Identification General

What is Middlebrook 7H10 used for?

Middlebrook 7H10 Agar is transparent agar-based medium for mycobacteria (vs egg-based LJ medium). Advantages: (1) Transparent - easier colony observation and counting, (2) Faster growth (2-3 weeks), (3) Better for drug susceptibility testing, (4) Can observe early growth. Requires OADC enrichment (Oleic acid, Albumin, Dextrose, Catalase) added after autoclaving. M. tuberculosis produces characteristic rough, dry, buff colonies. Used in clinical mycobacteriology labs for TB culture and sensitivity.
Related Products:

What is Lowenstein-Jensen Medium?

Lowenstein-Jensen (LJ) Medium is gold standard for Mycobacterium tuberculosis cultivation from clinical specimens. Egg-based medium contains: malachite green (inhibits non-mycobacteria), asparagine, glycerol. Prepare by: inspissation (solidification by heating at 85°C for 50 min rather than autoclaving). M. tuberculosis grows slowly (3-8 weeks), producing buff/cream rough colonies. Used for: TB diagnosis, drug susceptibility testing, mycobacterial identification. Slopes stored in screw-cap tubes. For isolation and cultivation of Mycobacterium species from clinical samples.
Related Products: