Microbiology Testing FAQs — Methods & Media | HiMedia USA

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What is Mueller-Hinton Agar used for?

Mueller-Hinton (MH) Agar is the standard medium for antimicrobial susceptibility testing (AST) by disk diffusion (Kirby-Bauer) method per CLSI guidelines. Formulation optimized for: (1) Reproducible results, (2) Consistent diffusion of antibiotics, (3) Minimal interference with antibiotics, (4) pH 7.2-7.4 (critical for some antibiotics). MH supports most clinically significant bacteria (not fastidious). For fastidious organisms, use MH + 5% sheep blood (for Streptococcus) or MH + blood + NAD/hemin (for Haemophilus). Plate depth must be 4mm (25mL per 100mm plate).
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