PCR Enhancers

PCR enhancer is an additive included in a PCR reaction that increases the specificity and yield of primary PCR amplification products. It is generally used in PCR reactions with G-C rich templates and/or with the PCR reactions amplifying complex or long DNA segments. PCR additives enhance PCRs through different mechanisms by stabilizing DNA polymerases and preserving their activity at high temperatures, by destabilizing mismatched primer-template complexes, by reducing the formation of secondary structure in GC-rich regions or/and by lowering strand separation temperatures and promoting DNA denaturation at the initial stage of PCR.