Microbiology Testing FAQs — Methods & Media | HiMedia USA

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What is LB Agar used for?

Luria Bertani (LB) Agar is standard medium for cultivation of E. coli and other Enterobacteriaceae in molecular biology and genetic engineering. Simple formulation (tryptone, yeast extract, NaCl) supports rapid E. coli growth. Used for: (1) Transformation experiments (with antibiotic selection), (2) Maintaining recombinant strains, (3) Plasmid preparation, (4) Blue-white screening (with X-Gal, IPTG), (5) Cloning procedures. Can add ampicillin, kanamycin, or other antibiotics for selection of transformed colonies. Incubate 16-18 hours at 37°C.
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