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Microbiology Testing FAQs — Methods & Media | HiMedia USA
What is m-Enterococcus Agar used for?
m-Enterococcus Agar is selective medium for enumeration of enterococci in water by membrane filtration per EPA Method 1600. Enterococci are fecal indicators more stable in seawater than E. coli. Contains: (1) Sodium azide - inhibits gram-negatives, (2) Triphenyltetrazolium chloride (TTC) - reduced to red formazan by enterococci. Enterococci produce red/pink colonies. Used for: (1) Marine/estuarine water quality, (2) Recreational beach water, (3) Wastewater monitoring. EPA recommends enterococci for saltwater beaches. Filter water → place on m-Enterococcus → incubate 48h at 41°C.
What makes mEnterococcus different from m-Enterococcus?
These are typically similar formulations - naming variation. Both are selective media for enterococci membrane filtration. Check specific product formulation. Standard m-Enterococcus contains sodium azide and TTC. mEnterococcus Agar (alternate formulation) may use bile esculin for differentiation instead of TTC. Both detect enterococci from water - preferred indicators for marine/coastal water quality per EPA. For isolation and enumeration of Enterococci in water, sewage, food and other materials by membrane filtration technique as well as direct plating of specimens
What is mCP Agar?
mCP Agar is selective medium for C. perfringens enumeration in water by membrane filtration. C. perfringens spores survive water treatment that kills indicators like E. coli, making it useful for detecting fecal contamination in treated water. Contains antibiotics (D-cycloserine, polymyxin B) and phenolphthalein diphosphate. C. perfringens produces yellow colonies. Requires anaerobic incubation 24h at 44°C. Used to assess sewage pollution and treatment effectiveness. For isolation and enumeration of Enterococci in water, sewage, food and other materials by membrane filtration technique as well as direct plating of specimens.
What is mFC Agar?
mFC Agar is selective medium for fecal coliform enumeration in water by membrane filtration at elevated temperature (44.5°C). Contains bile salts and aniline blue inhibiting non-fecal bacteria. Fecal coliforms produce blue colonies. Elevated temperature differentiates fecal coliforms (grow at 44.5°C) from environmental coliforms (don't grow). Used for: recreational water monitoring, drinking water, wastewater. Filter water, incubate membrane on mFC in water bath at 44.5±0.2°C for 24h. For detection and enumeration of faecal coliforms using membrane filtration technique at higher temperature (44.5°C).
What is standard Endo Agar?
Endo Agar (not M-Endo for membrane filtration) is selective differential medium for coliform detection in water by pour plate or surface spread method. Contains: (1) Lactose and basic fuchsin-sodium sulfite - differentiate lactose fermenters, (2) Sodium sulfite - partially selective against non-coliforms. Lactose-fermenting coliforms (E. coli) produce red colonies with golden metallic sheen (characteristic diagnostic). Non-lactose fermenters produce colorless colonies. Older method, largely replaced by membrane filtration (M-Endo) or chromogenic media. Still used in some water testing labs and teaching.