Microbiology Testing FAQs — Methods & Media | HiMedia USA

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What is Cetrimide Agar used for?

Cetrimide Agar is a selective medium for isolation of Pseudomonas aeruginosa from clinical, pharmaceutical, and water samples. Cetrimide (quaternary ammonium compound) selectively inhibits most bacteria except Pseudomonas species. P. aeruginosa produces: (1) Green-blue pigment (pyocyanin), (2) Fluorescent yellow-green pigment under UV (pyoverdin), (3) Sweet grape-like odor. Used for: (1) Burn wound infections, (2) Pharmaceutical water testing, (3) USP <61> identification, (4) Environmental monitoring. Incubate 24-48 hours at 35-37°C. View under UV (365nm) to confirm fluorescence. In accordance with the microbial limit testing by harmonized methodology of USP/EP/BP/JP/IP.
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