Escherichia coli

Escherichia coli and its recombinant strains, a versatile and widely used bacterium in research and biotechnology acts like the workhorse of the field, playing a crucial role in countless discoveries and advancements and needs a variety of media to thrive depending upon the intended use and application. Selecting the correct medium depends on the specific experiment, research goal, and Escherichia coli strain characteristics. HiGenoMB supplies enriched growth media for cultivation and maintenance of Escherichia coli and its recombinant strains. In molecular biology, selecting the most suitable growth media for your Escherichia coli strain for cultivation and maintenance is crucial for varying applications such as Protein Expression, Nucleic Acid Isolation, Cloning and Transformation, e.t.c. HiGenoMB offers media such as:

Rich Media

• Luria-Bertani (LB) broth/agar: The most common and versatile, providing all essential nutrients for rapid growth. Contains peptone, yeast extract, and NaCl.
• 2XYT broth: Similar to LB but with twice the yeast extract, promoting protein expression.

Minimal Media

• M9 Minimal Medium: Contains minimal nutrients like salts, carbon source (glucose), and trace elements. Useful for studying specific metabolic pathways or gene expression.

Selective Media

• Terrific Broth (TB): Used for protein expression, contains high levels of amino acids to support protein synthesis

Specialty Media

• SOC Medium: Used for recovery of transformed E. coli cells after plasmid introduction