Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
BHI Agar w/ 1% Agar, HiVeg™ (Brain Heart Infusion Agar w/ 1% Agar, HiVeg™)
A solid medium recommended for the cultivation of fastidious pathogenic bacteria, yeasts and moulds.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use:
Recommended for cultivation of fastidious pathogenic bacteria, yeasts and moulds.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ infusion | 10.00 |
| HiVeg™ special infusion | 7.50 |
| HiVeg™ peptone No. 3 | 10.000 |
| Dextrose (Glucose) | 2.000 |
| Sodium chloride | 5.000 |
| Disodium hydrogen phosphate | 2.500 |
| Agar | 10.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 47.0 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well before pouring. If desired, 20 units Penicillin and 40 µg Streptomycin per ml of medium may be added to make the medium selective for fungi.
Principle And Interpretation
BHI Agar w/ 1% Agar, HiVeg™ is similar in composition to Brain Heart Infusion Agar, with the difference being in the concentration of Agar. It is a highly nutritious and can support luxuriant growth of wide variety of microorganisms. It can be further enriched by the addition of blood or rendered selective by adding different antibiotics (1,2). It is a general purpose medium used for primary isolation of aerobic bacteria from clinical specimens. Addition of 50 mg/l chloramphenicol or 40mg/l streptomycin or a mixture of 50mg/l gentamicin and 50mg/l chloramphenicol along with 5-10% sterile defibrinated blood is often recommended for inhibition of bacteria and isolation of pathogenic systemic fungi.
A mixture of cycloheximide (0.5 g/l) and chloramphenicol (0.05 g/l) is also used for selective isolation of pathogenic fungi (incubation at 25-30°C for 1-2 weeks) (3). Some fungi may be inhibited on this medium with 10% sheep blood, gentamicin and chloramphenicol (4,5,6).
BHI Agar w/ 1% Agar, HiVeg™ is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ infusion, HiVeg™ special infusion and HiVeg™ peptone No. 3 used in the media serves as sources of carbon, nitrogen, vitamins, amino acids, along with essential growth factors. Dextrose is the energy source. Sodium chloride maintains the osmotic equilibrium of the medium while disodium phosphate buffers the medium. Defibrinated sheep blood added to the basal medium provides essential growth factors for the more fastidious fungal organisms.
Type of specimen
Please add specimens
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- As organisms differ in their nutritional requirements, some fastidious organisms may be inhibited or may show poor growth.
- Further biochemical tests must be carried out for complete identification.
Performance and Evaluation
Performace of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.0% Agar gel
Colour and Clarity of prepared medium
Basal medium: Light amber coloured clear to slightly opalescent gel. After addition of 5% v/v sterile defibrinated blood: Cherry red coloured, opaque gel forms in Petri plates.
Reaction
Reaction of 4.7% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. (If desired add 5% v/v sterile defibrinated blood)
| Organism | Inoculum (CFU) | Growth | Recovery | Growth w/ blood | Recovery w/ blood |
|---|---|---|---|---|---|
| Candida albicans ATCC 26790 | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
| Shigella flexneri ATCC 12022 (00126*) | 50-100 | luxuriant | >70% | luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | BHI Agar w/ 1% Agar, HiVeg™ (Brain Heart Infusion Agar w/ 1% Agar, HiVeg™) |
|---|---|
| SKU | MV211A |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Roseburg T. et al, 1944, J. Infect. Dis., 74:131. 2.Conant N. F., 1950, Diagnostic Procedures and Reagents, 3rd Ed., APHA Inc. 3.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williamsand Wilkins, Baltimore. 4.Creitz and Puckett, 1954, Am. J. Clin. Pathol., 24:1318. 5.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. 6.Ajello L., Georg L., Kaplan W. and Kaufman L., 1963, CDC Laboratory Manual for Medical Mycology, PHS PublicationNo. 994, U.S. Govt. Office, Washington, D.C. 7.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition. 8.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual ofClinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
Write Your Own Review
BHI w/0.1% Agar (Brain Heart Infusion w/ 0.1% Agar)
As low as
₹5,876.00
BHI Agar w/ 3.0% Agar (Brain Heart Infusion Agar w/ 3.0% Agar)
As low as
₹7,062.00
BHI Agar, Modified (Brain Heart Infusion Agar, Modified)
As low as
₹5,983.00
BHI Agar (Brain Heart Infusion Agar) (Special Infusion Agar)
As low as
₹1,103.00
BHI Agar w/ 1% Agar (Brain Heart Infusion Agar w/ 1% Agar)
As low as
₹5,983.00
Upsell Products
HiEncap™ BHI Agar (HiEncap™ Special Infusion Agar)
As low as
₹0.00
BHI Agar Slant w/ 5% Sheep Blood
As low as
₹1,280.00
BHI Agar Slant w/ 10% Sheep Blood, Chloramphenical and Gentamicin
As low as
₹1,410.00
BHI CC Agar Slant w/ 10% Sheep Blood and Gentamicin
As low as
₹1,581.00
BHI Agar Slant
As low as
₹1,139.00
BHI Agar
As low as
₹1,192.00
BHI Agar Plate
As low as
₹1,670.00
BHI Agar Plate w/ Blood
As low as
₹1,210.00
BHI Agar Plate w/ Vancomycin
As low as
₹997.00
BHI Blood Agar Plate w/ Vancomycin
As low as
₹1,316.00