Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for cultivation of fastidious pathogenic bacteria that are resistant to vancomycin particularly enterococci.
Composition
| Ingredients | g/L |
|---|---|
| HM infusion powder # | 12.500 |
| BHI powder | 5.000 |
| Proteose peptone | 10.000 |
| Dextrose (Glucose) | 2.000 |
| Sodium chloride | 5.000 |
| Disodium hydrogen phosphate | 2.500 |
| Agar | 15.000 |
| Vancomycin | 8mcg |
| 5% Sheep blood | 50 ml |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Calf brain infusion from
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
BHI Agar is highly nutritious and can support luxuriant growth of wide variety of microorganisms. It can be further enriched by the addition of blood or rendered selective by adding different antibiotics (1,2). Enterococci usually occur as the normal flora in the intestines of mammals. The presence of enterococci is an indication of faecal contamination (3). The increasing development of multiple resistance towards antibiotics particularly vancomycin by enterococci is a serious threat to the world (4). Vancomycin- resistant Enterococcus (VRE) is the name given to a group of bacterial species of the genus Enterococcus that are resistant to the antibiotic vancomycin. Vancomycin resistant Enterococci Agar is formulated as per the recommendations of Centre for Disease Control and Prevention (CDC) for the selective isolation of vancomycin resistant enterococci (5).
Proteose peptone and infusions used in the media serves as sources of carbon, nitrogen, vitamins, amino acids, along with essential growth factors. Dextrose is the energy source. Sodium chloride maintains the osmotic equilibrium of the medium while disodium phosphate buffers the medium. Defibrinated sheep blood added to the basal medium provides essential growth factors for the growth. Addition of Vancomycin helps in inhibiting gram positive organisms and selectively permits the growth of Vancomycin Resistant Enterococci.
Type of specimen
Clinical samples - Rectal swab, Stool sample
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (6,7).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Some intermediate strains may show poor growth due to nutritional variations and resistance to Vancomycin.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile BHI blood agar w/vancomycin in 90 mm disposable plates with smooth surface and absence of black particles/ cracks/bubbles
Colour of medium
Red coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plates.
pH
7.10-7.50
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Enterococcus faecalis (VRE)ATCC 51299 | 50-100 | luxuriant | >=50% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=103 | inhibited | 0% |
| Enterococcus faecalis ATCC 29212 (00087*) | >=103 | inhibited | 0% |
| Enterococcus faecium (VRE) ATCC 700221*) | 50-100 | luxuriant | >=50% |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | BHI Blood Agar Plate w/ Vancomycin |
|---|---|
| SKU | MP5382 |
| Customized Product Available | No |
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