Cell Biology

HAT Medium Supplement 50X, Liquid w/ 680.5 mg/litre Hypoxanthine, 8.8 mg/litre Aminopterin and 193.8 mg/litre Thymidine in Phosphate Buffered Saline

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As low as ₹9,841.00
HAT supplement contains Hypoxanthine, Aminopterin and Thymidine and is used for the preparation of selection medium for hybridoma. The myeloma cell is deficient in enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT) or thymidine kinase (TK), and cannot survive in selection medium containing hypoxanthine, aminopterin and thymidine. TCL072 is a sterile filtered 50X HAT medium supplement containing 680.5 mg/litre Hypoxanthine, 8.8 mg/litre Aminopterin and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

HT Medium Suplement 50X, Liquid w/ 680.5 mg/litre Hypoxanthine and 193.8 mg/litre Thymidine in Phosphate Buffered Saline

Sold by:
As low as ₹10,992.00
The specific action of aminopterin present in the HAT system is to inhibit the de novo DNA synthesis. Hence, it needs to be eliminated once the hybrids are selected and isolated. Aminopterin is diluted by several passages in hypoxanthine-thymidine (HT) supplemented medium for about 2-3 weeks. Once the de novo nucleoside-synthesis pathway has been reinstated, HT supplementation can be discontinued. TCL073 is a sterile filtered, cell culture tested 50X HT medium supplement containing 680.5 mg/litre Hypoxanthine and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

Giemsa Stain Solution

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Giemsa stain is a mixture of methylene blue, eosin, and azure B. It is specific for the phosphate groups of DNA and attaches itself to where there are high amounts of adenine thymine bonding. Giemsa stain is mainly used for staining of peripheral blood smears and specimens obtained from the bone marrow. Giemsa stain is also used in cytogenetics to stain the chromosomes and identify chromosomal aberrations. TCL083 is a sterile filtered concentrated solution of Giemsa stain.

HAT Medium Supplement 50X, Liquid w/ 680.5 mg/litre Hypoxanthine, 8.8 mg/litre Aminopterin and 193.8 mg/litre Thymidine in Phosphate Buffered Saline

Sold by:
As low as ₹9,841.00
HAT supplement contains Hypoxanthine, Aminopterin and Thymidine and is used for the preparation of selection medium for hybridoma. The myeloma cell is deficient in enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT) or thymidine kinase (TK), and cannot survive in selection medium containing hypoxanthine, aminopterin and thymidine. TCL072 is a sterile filtered 50X HAT medium supplement containing 680.5 mg/litre Hypoxanthine, 8.8 mg/litre Aminopterin and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

HT Medium Suplement 50X, Liquid w/ 680.5 mg/litre Hypoxanthine and 193.8 mg/litre Thymidine in Phosphate Buffered Saline

Sold by:
As low as ₹10,992.00
The specific action of aminopterin present in the HAT system is to inhibit the de novo DNA synthesis. Hence, it needs to be eliminated once the hybrids are selected and isolated. Aminopterin is diluted by several passages in hypoxanthine-thymidine (HT) supplemented medium for about 2-3 weeks. Once the de novo nucleoside-synthesis pathway has been reinstated, HT supplementation can be discontinued. TCL073 is a sterile filtered, cell culture tested 50X HT medium supplement containing 680.5 mg/litre Hypoxanthine and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

ß-Propiolactone

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β-Propiolactone is an organic compound of the lactone family. It is commonly used to sterilize vaccines, enzymes, tissue grafts, nutrient broths, blood plasma, water, milk, surgical instruments. Its sporicidal action is used against vegetative bacteria,pathologic fungi and viruses. It has been used as an intermediate in the production of acrylic acid and esters. It has also been used to inactivate viruses for use in vaccines for animals and human.

Sure Fetal Bovine Sera, Heat Inactivated, Gamma-Irradiated, Sterile Filtered ,US

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RM10951 is heat inactivated and gamma irradiated Fetal bovine serum. Heat inactivation is done to destroy heat labile components such as complement that can lead to complement mediated cell lysis. Complement proteins, antibodies and enzymes present in the serum are inactivated by heat inactivation. It is gamma irradiated with 25- 35kGy dose. The serum is collected from approved abbatoirs in USA. US Origin FBS is considered the world’s standard for quality and is one of the most widely used serum in the world.

Sure Fetal Bovine Sera, Gamma-Irradiated, Sterile Filtered , New Zealand

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RM10978 is gamma irradiated Fetal Bovine Serum. Gamma irradiation minimizes the risk of viruses in animal origin material as it reduces or eliminates wide range of viruses and mycoplasmas, which may be present in the serum after filtration, thus enhancing biological safety of the serum. It is gamma irradiated with 25- 35kGy dose. This serum has been collected from approved abattoirs in New Zealand. New Zealand is considered to be free of BSE and FMD and belongs to GBR 1 category and thus, assures the lowest possible viral risk.

With our scalable, broad ranging cell biology platform and support, HiMedia combines technological insight with world-class manufacturing

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Mammalian Cell Biology

The cell-biology Division of HiMedia labs set the foundation for our pioneering journey in biosciences.

Our Cell Biology division stands on a strong foundation of in-depth research technologies and methodologies which help to transform new ideas into manufacturing quality cell biology media & reagents, to further establish drug discovery and drug development assays or, produce recombinant proteins.

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We support all cell biology life
For Life is Precious
While humanity is currently challenged with incurable diseases and lack of remedies, with the reach and research of an established cell biology division, we are aiding to find newer therapeutic modalities in the field of Stem Cell Therapy, Regenerative Medicine, Targeted Gene Therapy, Recombinant Protein Production and Vaccines.
With our animal free alternatives, like Serum Free Media and Serum Replacement, we ensure the safety of end product and concomitantly helping reduce the carbon footprint.
 

Automation

  • Liquid handling
  • Automated cell counting
  • Biochemical analysis
  • High throughput screening

Media Development

  • Design of Experiments
  • Multi-level factorial screening and interaction analysis
  • Amino acid analysis

Customization

  • Formulation
  • Testing
  • Packaging

Process

  • Growth curve
  • Serial subculturing
  • Cloning efficiency
  • Planting efficiency
  • Endotoxin testing

Tools

  • 60+ Cell Lines
  • 6+ Primary Cells
  • 3+ Stem Cells

Methods

  • Particle size analysis
  • Critical Component analysis
  • Identification by IR
  • Impurity analysis
  • Trace elements and heavy metal analysis

Quality Management System

  • ISO9001:2015
  • ISO13485:2016
  • WHO-GMP
  • Kosher - Halal

Health & Environment Safety

  • Institutional Biosafety Committee in accordance with Department of Biotechnology (DBT) for use of recombinant cell clones

Tissue Processing

  • Institutional Ethics Committee (IEC) and Institutional Committee for Stem Cell Research (IC-SCR) in accordance with Indian Council of Medical Research (ICMR)
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