Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for the cultivation of fastidious pathogenic bacteria, yeasts and moulds from clinical and non clinical samples.
Composition**
| Ingredients | g/L |
|---|---|
| HM infusion powder # | 12.500 |
| BHI powder | 5.000 |
| Proteose peptone | 10.000 |
| Dextrose (Glucose) | 2.000 |
| Sodium chloride | 5.000 |
| Disodium hydrogen phosphate | 2.500 |
| Agar | 15.000 |
| Defibrinated Sheep Blood | 50.00 ml |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Calf brain infusion from
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
BHI Agar is highly nutritious and can support luxuriant growth of wide variety of microorganisms (1,2). It can be further enriched by the addition of blood or rendered selective by adding different antibiotics (3,4,5). It is a general purpose medium used for primary isolation of aerobic bacteria from clinical specimens.
Proteose peptone and infusions used in the media serves as sources of carbon, nitrogen, vitamins, amino acids, along with essential growth factors. Dextrose is the energy source. Sodium chloride maintains the osmotic equilibrium of the medium while disodium phosphate buffers the medium. Defibrinated sheep blood added to the basal medium provides essential growth factors for the more fastidious bacteria, yeasts and moulds (2,3).
Type of specimen
Clinical samples -all other types of specimens where fastidious organisms, slow growing fungi, actinomycetes are involved in infecion
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Since the medium is nonselective, specimens heavily contaminated with normal flora should be inoculated on selective media.
- Further biochemical tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile BHI Blood Agar in 90 mm disposable plate with smooth surface and absence of black particles/cracks/bubbles
Colour of medium
Cherry red coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plate
pH
7.20-7.60
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Haemolysis |
|---|---|---|---|---|
| Streptococcus pneumoniae ATCC 6303 | 50-100 | luxuriant | >=70% | alpha |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant | >=70% | beta |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | >=70% | beta |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00033*) | 50-100 | luxuriant | >=70% | beta |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | luxuriant | >=70% | beta |
| Escherichia coli ATCC 25922(00013*) | 50-100 | luxuriant | >=70% | None |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | BHI Agar Plate w/ Blood |
|---|---|
| SKU | MP5380 |
| Customized Product Available | No |
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