Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for recovery of pathogenic dimorphic fungi from clinical and non clinical specimens.
Composition
| Ingredients | g / L |
|---|---|
| HM infusion powder # | 12.500 |
| BHI powder | 5.000 |
| Proteose peptone | 10.000 |
| Dextrose (Glucose) | 2.000 |
| Sodium chloride | 5.000 |
| Disodium hydrogen phosphate | 2.500 |
| Sheep blood | 50.000ml |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Calf brain infusion from
Directions
Streak the test inoculum aseptically into the slant and incubate at appropriate conditions.
Principle And Interpretation
Brain Heart Infusion Agar is highly nutritious and can support luxuriant growth of wide variety of microorganisms. It is enriched by the addition of blood (1, 2). It is a general purpose medium used for primary isolation of aerobic bacteria from clinical specimens.
Proteose peptone and infusions used in the media serves as sources of carbon, nitrogen, vitamins, amino acids, along with essential growth factors. Dextrose is the energy source. Sodium chloride maintains the osmotic equilibrium of the medium while disodium phosphate buffers the medium. Defibrinated sheep blood added to the basal medium provides essential growth factors for the more fastidious fungal organisms.
Type of specimen
Clinical samples - Pathological samples from blood and body fluids
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement
- Further biochemical tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile BHI Agar Slant w/ 5% Sheep Blood in glass tube.
Colour of medium: Cherry red coloured slant
Quantity of medium: 8ml of medium in glass tube
Reaction: 7.20-7.60
Sterility Check: Passes release criteria
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Streptococcus pneumoniae ATCC 6303 | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | BHI Agar Slant w/ 5% Sheep Blood |
|---|---|
| SKU | SL180 |
| Customized Product Available | No |
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