Skip to the beginning of the images gallery

Baird Parker HiVeg™ Agar Base

0 Review

As low as ₹1,687.00

Availability: In stock

Only %1 left

SKU:

MV043

For isolation and enumeration of coagulase positive Staphylococci from food and other materials.

Description

Composition

Ingredients	Grams/Litre
HiVeg hydrolysate	10.0
HiVeg extract	5.0
Yeast extract	1.0
Glycine	12.0
Sodium pyruvate	10.0
Lithium chloride	5.0
Agar	20.0

Final pH (at 25°C) 7.0 ± 0.2

** Formula adjusted, standardized to suit performance parameters.

Directions

Suspend 63 grams in 950 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 50 ml concentrated Egg Yolk Emulsion (FD045) and 3 ml sterile 3.5% Potassium Tellurite solution (FD047) or 50 ml Egg Yolk Tellurite Emulsion (FD046) for identification of coagulase positive Staphylococci. Alternatively one vial of FD195 (Fibrinogen Plasma Trypsin Inhibitor Supplement) may be added per 90 ml of medium. For more selectivity of the medium one vial of FD069 (BP sulpha supplement) per 1000 ml may be added. Mix well before pouring.

Warning: Lithium Chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately.

Principle and Interpretation

HiVeg Baird Parker Agar Base is prepared by using vegetable peptones which are free from BSE/TSE risks. This medium is modification of medium developed by Baird-Parker (1,2) from the tellurite - glycine formulation of Zebovitz et al (3) for isolation of Staphylococcus aureus from foods. Sodium pyruvate protects injured cells and helps recovery. Lithium chloride and Potassium Tellurite inhibit most of contaminating microflora except Staphylococcus aureus. Glycine, pyruvate enhances growth of Staphylococcus. With the addition of egg yolk the medium becomes light yellow, opaque. Proteolytic bacteria produce a clear zone around colony in egg yolk containing media. A clear zone and grey-black colonies on this medium are diagnostic for coagulase positive Staphylococci. Upon further incubation, an opaque zone is developed around colonies, which can be due to lipolytic activity. This medium, like the conventional medium is found to be less inhibitory to Staphylococcus aureus than other media, at the same time being more selective (4, 5). However, identity of Staphylococcus aureus isolated on Baird-Parker HiVeg Agar must be confirmed with a coagulase reaction. Baird-Parker HiVeg Agar Base can also be used to detect coagulase activity by adding Fibrinogen Plasma Trypsin Inhibitor Supplement (FD195) dissolved in 10 ml sterile distilled water added to 90 ml sterile molten medium kept at 45-50°C (6). Mix well and pour into plates. On this medium coagulase positive Staphylococcal colonies are white to grey-black surrounded by an opaque zone of coagulase activity within 24-40 hours incubation at 35°C. Reduction in tellurite is necessary because of absence of egg yolk emulsion. This results in translucent agar and white to grey coloured colonies of Staphylococci. For quantitative results select 20 - 200 colonies. Count Staphylococcus aureus like colonies and test them for coagulase reaction. Report Staphylococcus aureus per gram of food.

Product Profile

Vegetable based (Code MV)	Animal based (Code M)
MV043	M043
HiVeg hydrolysate	Casein enzymic hydrolysate
HiVeg extract	Beef extract

Recommended for

Isolation and enumeration of coagulase positive Staphylococci

Reconstitution

Quantity on preparation: 63.0 g/l
(500g): 7.9 L
(100g): 1.58 L

pH (25°C)

7.0 ± 0.2

Supplement

Egg Yolk Emulsion (FD045) and 3.5% Potassium Tellurite Solution (FD047) / Egg Yolk Tellurite Emulsion (FD046) / Fibrnogen Plasma Trypsin Inhibitor Supplement (FD195)

Sterilization

121°C / 15 minutes.

Storage

Dry Medium Below 30°C, Prepared Medium 2-8°C.

Quality Control

Appearance of powder

Light yellow coloured may have slightly greenish tinge homogeneous, free flowing powder.

Gelling

Firm, comparable with 2.0% Agar gel.

Colour and Clarity

Basal medium yields light amber coloured, clear to slightly opalescent gel. With addition of Egg Yolk Tellurite Emulsion (FD046) yellow coloured, opaque gel forms in petri plates.

Reaction

Reaction of 6.3% w/v aqueous solution of basal medium is pH 7.0 ± 0.2 at 25°C.

Cultural Response

Cultural characteristics observed after an incubation at 35 - 37°C for 24 - 48 hours, with added Egg Yolk Tellurite Emulsion (FD046)

Organisms (ATCC)	Inoculum (CFU)	Growth	Recovery	Colour of Colony	Lecithinase
Bacillus subtilis (6633)	10²-10³	none-poor	<10%	dark brown matt	-
Escherichia coli (25922)	10²-10³	none-poor	<10%	large brown black	-
Micrococcus luteus (10240)	10²-10³	poor-good	<30%	very small*
Proteus mirabilis (25933)	10²-10³	good-luxuriant	>50%	brown-black
Staphylococcus aureus (25923)	10²-10³	good-luxuriant	>50%	grey-black shiny	+
Staphylococcus epidermidis (12228)	10²-10³	poor-good	<30%	black

Key:

+ = Positive clear zone around the colony.
* = negative No zone
in shades of brown black

Product Information

More Information
Product Name	Baird Parker HiVeg™ Agar Base
SKU	MV043
Product Type	HiVeg™
Physical Form	Powder
Origin	Animal Free (Veg)
Packaging type	HDPE
References	1. Baird-Parker A. C., 1962, J. Appl. Bacteriol., 25:12. 2.Baird-Parker A. C. and Davenport E., 1965, J. Appl. Bacteriol., 28:390. 3.Zebovitz E., Evans J. B. and Niven C.F., 1955, J. Bacteriol., 70:686 . 4.Tardio and Baer, 1971, J. Assoc. Off. Anal. Chem., 54:728. 5.Baer, 1971, J. Assoc. Off. Anal. Chem., 54:732. 6.Assoc. off. Anal. Chem., 1971, 54:401. 7.Horwitz (Ed.), 2000, Official methods of analysis of AOAC International, 17th Ed., Vol. I., AOAC International,Gaithersburg, MD. 8.The United States Pharmacopoeia, 2018, The United States Pharmacopoeial Convention. Rockville, MD. 9.International Organization for Standardization (ISO), 1983, Draft ISO/DIS 6888. 10.Smith B. A. and Baird-Parker A.C., 1964, J. Appl. Bacteriol., 27:78. 11. Beckers N. J. et al, 1984, Can. J. Microbiol., 30:470.1 2.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., WashingtonD.C. 13.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C. 14..Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C. 15.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition. 16.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.
Customized Product Available	No