Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Baird Parker Agar Medium (In accordance with IP 1996)
For isolation and enumeration of coagulase positive Staphylococci from food and other materials in accordance with Indian Pharmacopoeia.
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Intended Use
Baird Parker Agar Medium is recommended for the selective isolation and enumeration of coagulase positive Staphylococci from food and other materials in accordance with Indian Pharmacopoeia.
Composition
| Ingredients | Gms/Litre |
|---|---|
| Pancreatic digest of casein | 10.000 |
| Beef extract | 5.000 |
| Yeast extract | 1.000 |
| Glycine | 12.000 |
| Sodium pyruvate | 10.000 |
| Lithium chloride | 5.000 |
| Agar | 20.000 |
| pH after sterilization | 6.8±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 63 grams in 950 ml purified/ distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 115°C for 30 minutes or alternatively at 15lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 50 ml Egg Yolk Emulsion (FD045) and 10 ml sterile 1% Potassium Tellurite Solution (FD052). Mix well and pour into sterile Petri plates.
Warning: Lithium Chloride is harmful. Avoid all bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately.
Principle And Interpretation
This medium was first described in 1952. This medium was developed by Baird-Parker (1,2) from the Tellurite-glycine formulation of Zebovitz et.al. (3) for selective isolation of Staphylococcus aureus from foods. Staphylococcus species are common contaminants in food, dairy, pharmaceutical and cosmetics related products (9). This medium is recommended for microbial limit tests and to detect S.aureus. Baird Parker Agar Medium was reported to be the best medium for selective detection of coagulase positive and enterotoxigenic Staphylococcus (4). This medium was found to be less inhibitory to S.aureus than other media, at the same time being more selective (5, 6). Subsequently it was officially adapted by the AOAC and is also recommended in Indian Pharmacopoeia for use in Microbial limit test (7, 8).
Beef extract, yeast extract and pancreatic digest of casein provides essential mineral, vitamin and other growth requirements. Sodium pyruvate protects injured cells and helps recovery. Lithium chloride and potassium tellurite inhibit most of contaminating microflora except S.aureus. Glycine, pyruvate enhances growth of Staphylococcus. With the addition of egg yolk the medium becomes yellow and opaque.
Proteolytic bacteria produce a clear zone around colony in egg yolk containing media also known as Lecithinase reaction. A clear zone and grey-black colonies on this medium are diagnostic for coagulase positive Staphylococci. Upon further incubation, an opaque zone is developed around colonies, which can be due to lipolytic activity. Identity of S.aureus isolated on Baird-Parker Agar must be confirmed with a coagulase reaction. Growth of Proteus species can be inhibited by addition of sulphamethazene in this medium.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 2.0% agar gel.
Colour and Clarity of prepared medium
Basal medium: Yellow coloured clear to slightly opalescent gel. After addition of Egg Yolk Emulsion and Tellurite Emulsion: Yellow coloured opaque gel forms in Petri plates.
Reaction
After sterilization, reaction of 6.3% w/v aqueous solution. pH : 6.8±0.2
pH
6.60-7.00
Cultural Response
Growth Promotion is carried out in accordance with IP. Cultural response was observed after an incubation at 30-35°C for 24-48 hours. Recovery rate is considered as 100% for bacteria growth on Soyabean Casein Digest Agar.
Cultural Response
| Organism | Inoculum (CFU) | Growth | Lot value (CFU) | Recovery | Colour of colony | Lecithinase |
|---|---|---|---|---|---|---|
| Growth promoting | ||||||
| Staphylococcus aureus ATCC 6538 | 50-100 | luxuriant | 25-100 | >=50% | grey-black shiny | Positive, opaque zone around the colony |
| Additional Microbiological testing | ||||||
| Staphylococcus aureus ATCC 25923 | 50-100 | luxuriant | 25-100 | >=50% | grey-black shiny | Positive, opaque zone around the colony |
| Proteus mirabilis ATCC 25933 | 50-100 | good - luxuriant | 50 -100 | >=50 | brown - black | Negative |
| Micrococcus luteus ATCC 10240 | 50-100 | poor - good | 15-40 | 30-40% | shades of brown-black (very small) | Negative |
| Staphylococcus epidermidis ATCC 12228 | 50-100 | poor - good | 15-40 | 30-40% | black | Negative |
| Bacillus subtilis ATCC 6633 | 50-100 | none - poor | 0-10 | 0-10% | dark brown matt | Negative |
| Escherichia coli ATCC 8739 | 50-100 | none- poor | 0-10 | 0-10% | large brown black | Negative |
| Escherichia coli NCTC 9002 | 50-100 | none- poor | 0-10 | 0-10% | large brown black | Negative |
| Escherichia coli ATCC 25922 | 50-100 | none- poor | 0-10 | 0-10% | large brown black | Negative |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium between 2-8°C. Use before expiry date on the label.
| Product Name | Baird Parker Agar Medium (In accordance with IP 1996) |
|---|---|
| SKU | MM043 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Baird-Parker,A.C. 1962,J.Appl.Bact.,25: 12. |
| Customized Product Available | No |
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