Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for enumeration and cultivation of Lactobacilli in pharmaceutical preparations.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 5.000 |
| Yeast extract | 5.000 |
| Dextrose (Glucose) | 2.000 |
| Potassium dihydrogen phosphate | 0.500 |
| Dipotassium hydrogen phosphate | 0.500 |
| Magnesium sulphate | 0.300 |
| Sodium chloride | 0.010 |
| Manganese sulphate | 0.010 |
| Zinc sulphate | 0.0016 |
| Copper sulphate | 0.0016 |
| Cobalt sulphate | 0.0016 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 28.32 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Glucose Yeast Extract Agar is prepared according to the formula described by Evans and Niven (1) and Rogosa et.al. (4) and is used for enumeration and cultivation of Lactobacilli in pharmaceutical preparations.
The medium contains variety of salts like sulphates, phosphates to support the growth of Lactobacilli. Necessary nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and essential growth nutrients for Lactobacilli are provided by peptone and yeast extract. Glucose is the source of fermentable carbohydrate. The metallic salts are sources of ions essential for the replication of lactic acid bacteria.
Type of specimen
Pharmaceutical samples
Specimen Collection and Handling
For pharmaceutical samples follow appropriate techniques for handling specimens as per established guidelines (2,3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Light yellow to beige homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 2.83% w/v aqueous solution at 25°C. pH: 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Lactobacillus acidophilus ATCC 4356 (00098*) | 50-100 | good-luxuriant | >=50% |
| Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 (00102*) | 50-100 | good-luxuriant | >=50% |
| Lactobacillus casei ATCC 9595 | 50-100 | good-luxuriant | >=50% |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
Reference
- Evans and Niven, 1951, J. Bacteriol., 62:599.
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
- Rogosa M., Mitchell J. A. and Wiseman R. F., 1951, J. Bacteriol., 62 :132.
- Seppo Salminen, Atte von Wright and Arthur Ouweh and, Lactic Acid Bacteria., Microbiological and Functional Aspects, 3rd Ed., Marcel and Dekker. NY. Basel.
| Product Name | Glucose Yeast Extract Agar |
|---|---|
| SKU | M963 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Evans and Niven, 1951, J. Bacteriol., 62:599.2.Rogosa M., Mitchell J. A. and Wiseman R. F., 1951, J. Bacteriol., 62 :132.3.Seppo Salminen, Atte von Wright and Arthur Ouweh and, Lactic Acid Bacteria., Microbiological and Functional Aspects,3rd Ed., Marcel and Dekker. NY. Basel. |
| Customized Product Available | No |
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