Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
This medium is prepared by completely replacing animal based peptones with vegetable peptones. Recommended for testing motility of Listeria monocytogenes.
Composition**
ISO 11290 Specification - Listeria Motility Medium
| Ingredients | g/L |
|---|---|
| Enzymatic digest of casein | 20.000 |
| Enzymatic digest of animal tissues | 6.100 |
| Agar | 3.500 |
| Final pH (at 25°C) | 7.3±0.2 |
MV1215- Listeria Motility HiVeg® Medium
| Ingredients | g/L |
|---|---|
| HiVeg® hydrolysate | 20.000 |
| HiVeg® peptone | 6.100 |
| Agar | 3.500 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 29.6 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubed medium to cool in an upright position.
Principle And Interpretation
Bacterial motility is one of the important determinants in making a final species identification. Bacteria move by means of flagella, the number and location of which vary among different species. Semisolid media in tubes are most commonly employed for detecting motility. Motility media have agar concentration of 0.4% or less. The motility test is interpreted by making a macroscopic examination of medium for a diffused zone of growth flaring out from the line of inoculation. Listeria monocytogenes requires room temperature incubation before motility develops, since in some organisms; flagellar proteins develop more rapidly at lower temperatures (room temperature) such as in L. monocytogenes and Yersinia enterocolitica. Listeria Motility Medium is formulated in accordance with ISO Committee specification (1) for the determination of motility by L. monocytogenes. Listeria Motility HiVeg® Medium is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg® hydrolysate and HiVeg® peptone serves as a source of nitrogen and carbon compounds, long chain amino acids and vitamins as source of growth nutrients.
Type of specimen
Isolated Microorganisms
Specimen Collection and Handling
The motility of L.monocytogenes is best demonstrated by stab inoculating two tubes of semisolid medium and incubating one at room temperature (22-30°C). Motility is better observed at room temperature (2). An umbrella-like zone of growth 2 to 5 mm below the surface of the medium is characteristic of L. monocytogenes. Motility at 35°C incubation is either absent or extremely sluggish.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Well isolated colonies must be used for detection of motility.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Semisolid, comparable with 0.35% Agar gel.
Colour and Clarity of prepared medium
Light yellow coloured, clear to slightly opalescent gel forms in tubes as butts
Reaction
Reaction of 2.96% w/v aqueous solution at 25°C. pH: 7.3±0.2
pH
7.10-7.50
Cultural Response
Cultural characteristics observed after an incubation at 22-30°C for 48° 2hours.
| Organism (ATCC) | Motility |
|---|---|
| Listeria monocytogenes ATCC 19117 | positive, growth away from stabline causing turbidity |
| Listeria monocytogenes serovar 1 ATCC 19112 | positive, growth away from stabline causing turbidity |
| Listeria monocytogenes serovar 4b ATCC 13932 (00021*) | positive, growth away from stabline causing turbidity |
| Listeria monocytogenes serovar l/2a ATCC 35152 (00109*) | positive, growth away from stabline causing turbidity |
| Listeria innocua ATCC 33090 (00017*) | positive, growth away from stabline causing turbidity |
| Listeria ivanovii subsp. ivanovii serovar 5 ATCC 19119 (00018*) | positive, growth away from stabline causing turbidity |
| Listeria grayi ATCC 19120 | positive, growth away from stabline causing turbidity |
| Listeria seeligeri ATCC 35967 | positive, growth away from stabline causing turbidity |
| Listeria welshimeri ATCC 43549 | positive, growth away from stabline causing turbidity |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
Reference
- Microbiology of the food chain Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 2, Enumeration method; ISO 11290-2:2017.
- Bailey and Scotts Diagnostic Microbiology, 1986, 7th Ed., The C.V. Mosby Co., St. Louis.
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
| Product Name | Listeria Motility HiVeg™ Medium |
|---|---|
| SKU | MV1215 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. International Organization for Standardization (ISO), 1993, Draft ISO/DIS 10560. |
| Customized Product Available | No |
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