Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for detection of bacterial motility.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptose | 10.000 |
| Sodium chloride | 5.000 |
| Agar | 5.000 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Motility Test Agar is a ready to use solid media in glass bottle. The medium is pre-sterilized, hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium in tubes as butts /slants or in plates as desired and allow to solidify. If on plate, either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically.
Principle And Interpretation
Bacterial motility can be observed directly on microscopic slide or it can be visualized on motility media having agar concentration of 0.4% or less (4). Use of such semisolid media to observe or detect motility was reported by Tittsler and Sandholzer (6). Motility Test Medium is a modification of their formulation. Motility can be visualized as a diffused zone of growth flaring out from the line of inoculation (4). Hanging-drop technique in motility tests has practical difficulties, which is efficiently eliminated by use of culture-based methods using semi-solid media, as in semisolid media; the results obtained are macroscopic and cumulative.
Tryptose serve as a source of essential growth nutrients required for bacterial metabolism. Sodium chloride maintains the osmotic equilibrium of the medium. Small amount of agar helps to create a semisolid medium.
Bacterial motility can be observed directly by examination of the tubes following incubation. Inoculation is done by stabbing through the centre of the medium. Incubate at appropriate temperature for 18-40 hours. Non-motile organisms grow only along the line of inoculation whereas motile organisms grow away from the line of inoculation or may show growth even throughout the medium. All weak or equivocal motility results should be confirmed by flagellum stain or by direct wet microscopy (hanging drop) (1,5).
Type of specimen
Isolated microorganism
Specimen Collection and Handling:
With inoculating needle, stab centre of medium to approximately one-half of depth. (4) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- 1.Growth from an 18-24 hr pure culture should be used. (5)
- 2. All weak or equivocal motility results should be confirmed by flagellum stain or by direct wet microscopy (hanging drop) (1,5).
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Motility Test Agar in glass bottle.
Colour medium
Light yellow coloured
Quantity of medium
100 ml of medium in glass bottle.
Reaction
7.00-7.40
Sterility Test
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Motility |
|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) |
50-100 | luxuriant | positive, growth away from stabline causing turbidity |
| # Klebsiella aerogenes ATCC 13048 (00175*) |
50-100 | luxuriant | positive, growth away from stabline causing turbidity |
| Klebsiella pneumoniae ATCC 13883 (00097*) |
50-100 | luxuriant | negative, growth along the stabline, surrounding medium remains clear |
| Salmonella Enteritidis ATCC 13076 (00030*) |
50-100 | luxuriant | positive, growth away from stabline causing turbidity |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) |
50-100 | luxuriant | negative, growth along the stabline, surrounding medium remains clear |
Key: (*) Corresponding WDCM numbers.
(#) Formerly known as Enterobacter aerogenes
Storage and Shelf Life
On receipt store between 15-25°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Motility Test Agar |
|---|---|
| SKU | SM260 |
| Customized Product Available | No |
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