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Plate Count HiVeg™ Agar (Standard Methods HiVeg™ Agar)

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SKU:

MV091

For determination of plate counts of microorganisms in food, water, waste water and clinical samples.

Description

Intended use

It is recommended for the determination of plate counts of micro-organisms in food, water, and waste water.

Composition**

Ingredients	g/L
HiVeg® hydrolysate	5.000
Yeast extract	2.500
Dextrose (Glucose)	1.000
Agar	15.000
Final pH (at 25°C)	7.0±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 23.5 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.

Principle And Interpretation

Plate Count Agar is formulated as described by Buchbinder et al (1) which is recommended by APHA (2,3,4) and FDA (5). Plate Count HiVeg® Agar is same as Plate Count Agar except that the animal peptones are completely replaced with vegetable peptones to avoid the BSE/TSE risks associated with animal peptones.

HiVeg® hydrolysate provides nitrogenous and carbonaceous compounds, long chain amino acids, and other essential nutrients. Yeast extract supplies Vitamin B complex. APHA recommends the use of pour plate technique. The samples are diluted and appropriate dilutions are added in Petri plates. Sterile molten agar is added to these plates and plates are rotated gently to ensure uniform mixing of the sample with agar. The poured plate count method is preferred to the surface inoculation method, since it gives higher results. Plate Count HiVeg® Agar is also suitable for enumerating bacterial count of sterile rooms.

Type of specimen

Food and dairy samples; Water samples

Specimen Collection and Handling:

For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3,4).

For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards(2).

After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions :

Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets

Limitations :

This medium is general purpose medium and may not support the growth of fastidious organisms.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance
Cream to yellow homogeneous free flowing powder

Gelling
Firm, comparable with 1.5% Agar gel

Colour and Clarity of prepared medium

Light yellow coloured clear to slightly opalescent gel forms in Petri plates

Reaction

Reaction of 2.35% w/v aqueous solution at 25°C. pH : 7.0±0.2

pH

6.80-7.20

Cultural Response

Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 48 hours.

Organism	Inoculum (CFU)	Growth	Recovery
*Bacillus spizizenni ATCC 6633 (00003)	50-100	luxuriant	>=70%
Enterococcus faecalis ATCC 29212 (00087*)	50-100	luxuriant	>=70%
Escherichia coli ATCC 25922 (00013*)	50-100	luxuriant	>=70%
Lactobacillus casei ATCC 9595	50-100	luxuriant	>=70%
Staphylococcus aureus subsp. aureus ATCC 25923 (00034*)	50-100	luxuriant	>=70%
Streptococcus pyogenes ATCC 19615	50-100	luxuriant	>=70%

Key: *Corresponding WDCM numbers

**Formerly known as Bacillus subtilis subsp. spizizenii.

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).

Product Information

More Information
Product Name	Plate Count HiVeg™ Agar (Standard Methods HiVeg™ Agar)
SKU	MV091
Product Type	HiVeg™
Physical Form	Powder
Origin	Animal Free (Veg)
Packaging type	HDPE
References	1. Buchbinder L., Baris Y., Aldd E., Reynolds E., Dilon E., Pessin V., Pincas L. and Strauss A., 1951, Publ. Hlth. Rep., 66:327. 2.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C. 3.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C. 4.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water andWastewater, 23rd ed., APHA, Washington, D.C. 5.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC. 6.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition. 7.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.
Customized Product Available	No