Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended for the determination of plate counts of microorganisms in food, water, waste water samples.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 5.000 |
| Yeast extract | 2.500 |
| Dextrose (Glucose) | 1.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Plate Count Agar is formulated as described by Buchbinder et al (1) which is recommended by APHA (2,3,4) and FDA (5). Tryptone provides nitrogenous and carbonaceous compounds, long chain amino acids, and other essential nutrients. Yeast extract supplies Vitamin B complex. APHA recommends the use of pour plate technique. The samples are diluted and appropriate dilutions are added in Petri plates. Sterile molten agar is added to these plates and plates are rotated gently to ensure uniform mixing of the sample with agar. The poured plate count method is preferred to the surface inoculation method, since it gives higher results. Plate Count Agar is also suitable for enumerating bacterial count of sterile rooms.
Type of specimen
Food and dairy samples; Water samples
Specimen Collection and Handling:
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3,4).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred to individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further serological and biochemical testing is required for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
AppearanceSterile Nutrient Agar in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles
Colour of mediumLight amber coloured medium
Quantity of medium25 ml of medium in 90 mm disposable plates.
pH6.80-7.20
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| ** Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant | >=70% |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% |
| Lactobacillus rhamnosus ATCC 9595 | 50-100 | luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | >=70% |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant | >=70% |
Key: *Corresponding WDCM numbers.
**Formerly known as Bacillus subtilis subsp. spizizenii
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Plate Count Agar Plate |
|---|---|
| SKU | MP091 |
| Customized Product Available | No |
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