Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended use
Recommended for determination of plate counts of microorganisms in foods, water and wastewater and inactivation of ẞ-lactam antibiotics.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 5.000 |
| Yeast extract | 2.500 |
| Dextrose (Glucose) | 1.000 |
| Agar | 15.000 |
| ẞ-Lactamase | 330.000IU |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Plate Count Agar is formulated as described by Buchbinder et al (1) which is recommended by APHA (2,3,4) and FDA (5). Tryptone provides nitrogenous and carbonaceous compounds, long chain amino acids, and other essential nutrients. Yeast extract supplies Vitamin B complex. ẞ-Lactamase inactivates of ẞ lactam antibiotics. APHA recommends the use of pour plate technique. The samples are diluted and appropriate dilutions are added in Petri plates. Sterile molten agar is added to these plates and plates are rotated gently to ensure uniform mixing of the sample with agar. The poured plate count method is preferred to the surface inoculation method, since it gives higher results. Plate Count Agar is also suitable for enumerating bacterial count of sterile rooms.
Type of specimen
Environmental sampling
Specimen Collection and Handling:
For Environmental sampling, follow appropriate techniques for sample collection and processing as per guidelines (6,7). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Soyabean Casein Digest Agar w/ beta-lactamase I in 90mm disposable plates with smooth surface & absence of black particles/cracks/bubbles
Colour
Light yellow coloured medium
Quantity of Medium
25ml of medium in 90 mm plate
Sterility Check
Passes release criteria
pH
7.10-7.50
Concentration
Concentration of Penicillinase added: 330 IU/lit of media (approximately 2,00,000 LU/lit)
Cultural Response
Growth Promotion Test of as such plates was carried out and growth was o bserved after incubation at 30-35°C for < = 3 days. Simultaneously growth promotion test was carried out on plates which were seeded with Recovery rate is considered 100% for bacteria 100 mcg/0.1ml of Benzyl Penicillin.
| Organism | Inoculum (CFU) | Growth | Recovery | Growth w/ antibiotic | Recovery w/ antibiotic |
|---|---|---|---|---|---|
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
| Staphylococcus aureus ATCC 29213 (00131*) | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 20-30°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Total Plate Count Agar Plate w/ β-Lactamase |
|---|---|
| SKU | MP1810 |
| Customized Product Available | No |
Write Your Own Review