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Brilliant Green Agar Base w/ Phosphates

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M971S

Recommended for the selective isolation of bacteria responsible for food poisoning as recommended by BIS committee under the specifications IS:5887 (Part III)-1999.

Description

Composition**

Ingredients	Gms/Litre
Peptic digest of animal tissue	10.000
Meat extract	5.000
Yeast extract	3.000
Lactose	10.000
Sucrose	10.000
Disodium phosphate	1.000
Monosodium phosphate	0.600
Phenol red	0.090
Brilliant green	0.0047
Agar	12.000
Final pH (at 25°C)	7.0±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 52 grams in 1000 ml distilled water. Heat with occasional agitation and bring just to the boil to dissolve the medium completely. DO NOT AUTOCLAVE. For maximum recovery, Sulpha Supplement (FD068) may be aseptically added. Cool to 50°C. Mix well and pour into sterile Petri plates.

Principle And Interpretation

This medium is formulated as per the recommendation of Rijks Institute Voorde Volksgezondheld (National Institute for Public Health), Utrecht (1,2). It is widely accepted and used in ISO Standards (3,4,5) because of its improved performance with respect to recovery of smaller numbers of Salmonella species, inhibition of Escherichia coli, Proteus species and Pseudomonas species (6). It is also recommended by BIS as a solid selective medium for the detection of Salmonella from food (7). As per BIS, the growth from Fluid Selenite Cystine Broth (M0251) and Modified Rappaport Vassiliadis Medium (M11371) is subcultured on Brilliant Green Agar with phosphates (M971S).

Typical colonies from the plates are used for further confirmation. The medium can be further supplemented with sulphacetamide (1 g/l) and sodium mandelate (0.25 g/l) to inhibit contaminating microorganisms when the sample is suspected to be containing large number of competing organisms along with Salmonella species (8).

Quality Control

Appearance: Light yellow to pink coloured homogeneous free flowing powder

Gelling: Firm, comparable with 1.2% Agar gel.

Colour and Clarity of prepared medium: Greenish brown coloured clear to slightly opalescent gel forms in Petri plates.

Reaction: Reaction of 5.2% w/v aqueous solution at 25°C. pH: 7.0±0.2

pH: 6.80-7.20

Cultural Response

Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.

Cultural Response

Organism	Inoculum (CFU)	Growth	Recovery	Colour of colony
Escherichia coli ATCC 25922	>=10³	inhibited	0%
Proteus vulgaris ATCC 13315	50-100	none-poor	<=10%	red
Pseudomonas aeruginosa ATCC 10145	50-100	none-poor	<=10%	red
Salmonella Enteritidis ATCC 13076	50-100	luxuriant	>=50%	bright red
Salmonella Typhimurium ATCC 14028	50-100	luxuriant	>=50%	bright red

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

Reference

Edel W. and Kampelmacher E.H., 1969, Bull. W.H.O., 41:297.
Edel W. and Kampelmacher E.H., 1969, Bull. W.H.O., 39:487.
Anon, 1975, International Organization for Standardization, Meat and Meat products. Ref. Method, ISO:3565.
Anon, 1981, International Organization for Standardization, Microbiology Ref. Methods, ISO: 6579.
Anon, 1985, International Organization for Standardization, Milk and Milk Products, Ref. Method, ISO : 6785.
Read R. B. and Reyes A.L., 1968, Appl. Microbiol., 16:746.
Bureau of Indian Standards IS: 5887 (Part 3) 1999.
Watson U.C. and Walker A.P., 1978, J. Appl. Bact., 45:195.

Product Information

More Information
Product Name	Brilliant Green Agar Base w/ Phosphates
SKU	M971S
Product Type	Regular
Physical Form	Powder
Origin	Animal
Packaging type	HDPE
References	1. Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C.2.Edel W. and Kampelmacher E. H., 1969, Bull. W.H.O., 41:297.3.Edel W. and Kampelmacher E. H., 1969, Bull. W.H.O., 39:487.4.Anon, 1975, International Organization for Standardization, Meat and Meat products Ref. Method, ISO: 3565.5.Anon, 1981, International Organization for Standardization, Microbiology Ref. Methods, ISO: 6579.6.Anon, 1985, International Organization for Standardization, Milk and Milk Products; Ref. Method, ISO: 6785.7.Read R. B. and Reyes A. L., 1968, Appl. Microbiol., 16:746.8.Watson U. C. and Walker A. P., 1978, J. Appl. Bacteriol. 45:195.
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