• Home
  • Brilliant Green Agar Base w/ Phosphates
main product photo

Brilliant Green Agar Base w/ Phosphates

0 Review
As low as ₹6,230.00
Availability: In stock
Only %1 left
SKU:
M971
For selective isolation of Salmonellae while inhibiting Escherichia coli, Proteus and Pseudomonas species.

Intended Use

Recommended for selective isolation of Salmonellae while inhibiting Escherichia coli, Proteus and Pseudomonas species.

Composition

Ingredients g/L
Peptone 10.000
HM peptone B # 5.000
Yeast extract 3.000
Lactose 10.000
Sucrose 10.000
Disodium hydrogen phosphate 1.000
Sodium dihydrogen phosphate 0.600
Phenol red 0.090
Brilliant green 0.0047
Agar 12.000
Final pH (at 25°C) 6.9±0.2

**Formula adjusted, standardized to suit performance parameters

# Equivalent to Beef extract

Directions

Suspend 25.84 grams in 500 ml purified/distilled water. Heat with occasional agitation and bring just to the boil to dissolve the medium completely. DO NOT AUTOCLAVE. For more selectivity and maximum recovery aseptically add the rehydrated contents of one vial of S Selective Supplement (FD068). Cool to 45-50°C. Mix well and pour into sterile Petri plates.

Principle And Interpretation

Salmonella species cause many types of infections, from mild self-limiting gastroenteritis to life threatening typhoid fever. The most common form of Salmonella disease is self-limiting gastroenteritis with fever lasting less than 2 days and diarrhoea lasting less than 7 days (1).

Brilliant Green Agar Base w/phosphates is formulated as per the recommendation of Rijks Institute Voorde Volksgezondheld (National Institute for Public Health), Utrecht (2,3). It is also recommended by the ISO Committee (4,5,6), because of its improved performance with respect to recovery of smaller numbers of Salmonella species, inhibition of Escherichia coli, Proteus species and Pseudomonas species (7).

The medium contains peptone, HM peptone B and yeast extract as sources of carbon, nitrogen, vitamins, amino acids and essential nutrients. The two sugars namely lactose and sucrose serve as energy sources. Fermentation of lactose and / or sucrose in the medium results in the formation of acidic pH which is detected by phenol red indicator. Phosphates (M971) buffer the medium. Brilliant green helps to inhibit the contaminating microflora. The medium can further supplemented with sulphacetamide (1g/l) and sodium mandelate (0.25g/l) to inhibit contaminating microorganisms when the sample is suspected to contain large number of competing organisms along with Salmonella species (8).

Brilliant Green Agar w/Phosphates being highly selective is recommended to be used along with a less inhibitory medium to improve the chances of recovery. Often cultures are enriched in Selenite Cystine Broth (M025) or Tetrathionate Broth (M032). These enriched cultures are then isolated simultaneously on Brilliant Green Agar Base (M016/M971), SS Agar (M108), Bismuth Sulphite Agar (M027) and MacConkey Agar (M081).

Type of specimen

Clinical: faeces; Food and dairy samples; Water samples

Specimen Collection and Handling

For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (9,10,11).

For clinical samples follow appropriate techniques for handling specimens as per established guidelines (13,14). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (12).

After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions

In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations

  1. Though this medium is selective for Salmonella other species of Enterobacteriaceae may grow.
  2. Further confirmation has to be carried out on presumptive Salmonella isolates.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance
Light yellow to pink homogeneous free flowing powder.

Gelling
Firm, comparable with 1.2% Agar gel.

Colour and Clarity of prepared medium
Greenish brown coloured clear to slightly opalescent gel forms in Petri plates.

Reaction
Reaction of 5.2% w/v aqueous solution at 25°C. pH: 6.9±0.2

pH
6.70-7.10

Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.

Organism Inoculum (CFU) Growth Recovery Colour of colony
Escherichia coli ATCC 25922 (00013*) >=104 inhibited 0%
$ Proteus hauseri ATCC 13315 50-100 none-poor <=10% red
Pseudomonas aeruginosa ATCC 10145 (00024*) 50-100 none-poor <=10% red
Salmonella Enteritidis ATCC 13076 (00030*) 50-100 luxuriant >=50% bright red
Salmonella Typhimurium ATCC 14028 (00031*) 50-100 luxuriant >=50% bright red

Key: (*) Corresponding WDCM numbers.

$ Formerly known as Proteus vulgaris

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (13,14).

Reference

  1. Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, 8th Ed., American Society for Microbiology, Washington, D.C.
  2. Edel W. and Kampelmacher E. H., 1969, Bull. W.H.O., 41:297.
  3. Edel W. and Kampelmacher E. H., 1969, Bull. W.H.O., 39:487.
  4. Anon, 1975, International Organization for Standardization, Meat and Meat products Ref. Method, ISO: 3565.
  5. Anon, 1981, International Organization for Standardization, Microbiology Ref. Methods, ISO: 6579.
  6. Anon, 1985, International Organization for Standardization, Milk and Milk Products; Ref. Method, ISO: 6785.
  7. R. B. and Reyes A. L., 1968, Appl. Microbiol., 16:746.
  8. Watson U. C. and Walker A. P., 1978, J. Appl. Bacteriol. 45:195.
  9. Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.
  10. Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C.
  11. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington D.C.
  12. Lipps WC, Braun-Howland EB, Baxter TE,eds. Standard methods for the Examination of Water and Wastewater, 24th ed. Washington DC:APHA Press; 2023.
  13. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
  14. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
More Information
Product Name Brilliant Green Agar Base w/ Phosphates
SKU M971
Product Type Regular
Physical Form Powder
Origin Animal
Packaging type HDPE
References 1. Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C.2.Edel W. and Kampelmacher E. H., 1969, Bull. W.H.O., 41:297.3.Edel W. and Kampelmacher E. H., 1969, Bull. W.H.O., 39:487.4.Anon, 1975, International Organization for Standardization, Meat and Meat products Ref. Method, ISO: 3565.5.Anon, 1981, International Organization for Standardization, Microbiology Ref. Methods, ISO: 6579.6.Anon, 1985, International Organization for Standardization, Milk and Milk Products; Ref. Method, ISO: 6785.7.Read R. B. and Reyes A. L., 1968, Appl. Microbiol., 16:746.8.Watson U. C. and Walker A. P., 1978, J. Appl. Bacteriol. 45:195.
Customized Product Available No
Write Your Own Review
You're reviewing:Brilliant Green Agar Base w/ Phosphates
Your Rating

Related Products

Upsell Products