Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Glucose Salt Teepol Broth (Twin pack)
Recommended for enrichment of Vibrio parahaemolyticus and marine isolates. It is recommended by BIS committee under the specifications IS:5887 (Part V)-1976, Reaffirmed 2005.
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Enrichment#CC293D
Composition
| Ingredients | Gms / Litre |
|---|---|
| Part A | |
| Peptic digest of animal tissue | 10.000 |
| Meat extract | 3.000 |
| Sodium chloride | 30.000 |
| Glucose | 5.000 |
| Methyl violet | 0.002 |
| Part B | |
| Teepol | 4.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 48 grams of Part A in 1000 ml distilled water containing 4 ml of Part B. Heat gently to dissolve the medium completely.
When double strength broth is required the ingredients in double amount are dissolved in 1000 ml water. Dispense in tubes as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Glucose Salt Teepol Broth is used to enrich Vibrio parahaemolyticus from sea foods and also used to enumerate the bacteria by MPN technique (1). Present formulation is recommended by BIS (2) for enrichment of Vibrio cholerae and other Vibrios responsible for food poisoning.
Peptic digest of animal tissue and meat extract provide essential nitrogenous nutrients and high percentage of sodium chloride (3%) helps for the better enrichment of halophilic Vibrio parahaemolyticus . Glucose is utilized by the organism while teepol inhibits the migration of halophilic organisms and the growth of the gram-positive organisms. After overnight incubation at 35 ± 2°C, a loopful of culture from top 1 cm of the broth showing growth is streaked onto TCBS Agar (M870S). Vibrio parahaemolyticus colonies on TCBS Agar appear as round, green or bluish measuring 2-3 mm in diameter, while Vibrio alginolyticus colonies are larger and yellow coloured. These colonies are further identified by biochemical characterization.
Quality Control
Appearance: Part A: Cream to yellow homogeneous free flowing powder Part B: Colourless viscous liquid
Colour & Clarity of Medium: Purple coloured clear solution with avery slight precipitate
Reaction: Reaction of 4.8% w/v aqueous solution with 0.4% Teepol at 25°C. pH: 7.4±0.2
pH: 7.20-7.60
Cultural response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Vibrio alginolyticus ATCC 17749 | 50-100 | good-luxuriant |
| Vibrio parahaemolyticus ATCC 17802 | 50-100 | good-luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Glucose Salt Teepol Broth (Twin pack) |
|---|---|
| SKU | M621S |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Vanderzant C. and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination of Foods,3rd Ed., APHA, Washington, D.C. 2.Thompson F. L., T. Iida and Swings J., 2004, Biodiversity of Vibrios, Microbiol. Mol. Biol. Rev., 68: 403-431. 3.Fujino T., Okuno Y., Nakada D., Aoyama A., Fukai K., Mukai T. and Ueho T., 1953, Med. J. Osaka Univ., 4:299-304. 4.Akiyama S., Takizawa K., and Obara Y., 1964, Ann. Rep. Kanagawa Pref. Inst. Public Health, 13:7 |
| Customized Product Available | No |
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