Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Saline Nutrient Agar is used for the cultivation of Vibrio parahaemolyticus from food products or animal feeding products.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 5.000 |
| Meat extract | 3.000 |
| Sodium chloride | 30.000 |
| Agar | 15.000 |
pH after sterilization (at 25°C): 8.50±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 53.0 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour in sterile Petri plates.
Principle And Interpretation
Vibrio parahaemolyticus is a halophilic estuarine organism. This organism can be isolated from a variety of sea food product and marine environments. The organism, when isolated from fresh sea food, is usually found in low number and is sensitive to refrigeration and heat.
Saline Nutrient Agar is recommended by ISO 8914:1990 (1) for isolating and enumerating Vibrio parahaemolyticus from food and animal feed.
Peptone and meat extract provide nitrogen compounds, growth factors and vitamins for the growth of Vibrio parahaemolyticus. High sodium chloride content and alkaline pH of the medium provides conditions that facilitate easy growth of Vibrio parahaemolyticus, while restricting the growth of most gram-negative microorganisms.
For isolation and confirmation of Vibrio parahaemolyticus, five typical colonies from Thiosulfate Citrate Bile Sucrose Agar (TCBS) or Triphenyltetrazolium Chloride Soya Tryptone Agar (TSAT) are subculture onto Saline Nutrient Agar followed by biochemical confirmation.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Light yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 5.3% w/v aqueous solution after sterilization at 25°C. pH : 8.5 ±0.2
pH: 8.30 - 8.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18- 24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Vibrio parahaemolyticus ATCC 17802 | 50-100 | good-luxuriant | >=70% |
| Vibrio cholerae ATCC 15748 | 50-100 | good-luxuriant | >=70% |
| Escherichia coli ATCC 25922 | 50-100 | good-luxuriant | >=70% |
| Enterobacter aerogenes ATCC 13048 | 50-100 | good-luxuriant | >=70% |
Storage and Shelf Life
Store below 30°C in tightly closed container and prepared medium at 2-8°C. Use before expiry date on the label.
Reference
- Indian Standard, Microbiology- General Guidance for the Detection of Vibrio parahaemolyticus. ISO 8914:1990.
| Product Name | Saline Nutrient Agar |
|---|---|
| SKU | M1776 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Indian Standard, Microbilogy- General Guidance for the Detection of Vibrio parahaemolyticus. ISO 8914:1990. |
| Customized Product Available | No |
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