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HiEncap™ Sabouraud Dextrose Agar

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SKU:

EC063CCL

For cultivation of yeasts, moulds and aciduric microorganisms.

Description

Intended Use

Recommended for cultivation of yeasts, moulds and aciduric microorganisms.

Composition

Ingredients	Gms/Litre
Dextrose (Glucose)	40.000
Mycological peptone	10.000
Agar	15.000
Final pH (at 25°C)	5.6±0.2

Formula adjusted, standardized to suit performance parameters

Directions

Each capsule contains 16.25 grams of medium. Suspend 1 capsule in 250 ml (4 capsules in 1000ml) distilled or purified water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.

Principle And Interpretation

Sabouraud Dextrose Agar is Carlier's modification (3) of the formulation described by is a modification of Sabouraud Dextrose Agar which is described by Sabouraud (7) for the cultivation of fungi (yeasts, moulds), particularly useful for the fungi associated with skin infections. This medium is also employed to determine microbial contamination in food, cosmetics, and clinical specimens (2).

Mycological Peptone provides nitrogenous compounds. Dextrose provides an energy source. High dextrose concentration and low pH favours fungal growth and inhibits contaminating bacteria from test samples (6).

Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet. For heavily contaminated samples, the plate must be supplemented with inhibitory agents for inhibiting bacterial growth with lower pH.

Type of specimen

Clinical samples: skin scrapings, Food samples; Cosmetics.

Specimen Collection and Handling

For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5). For food samples, follow appropriate techniques for sample collection and processing as per guidelines (1,4,8). After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions:

In Vitro diagnostic use. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations

For heavily contaminated samples, the plate must be supplemented with inhibitory agents for inhibiting bacterial growth with lower pH.
Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet.
Further biochemical tests should be carried out for confirmation.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance: Gelatin capsule containing cream to yellow coloured granular media

Gelling: Firm, comparable with 1.5% Agar gel

Colour and Clarity of prepared medium: Light amber coloured clear to slightly opalescent gel forms in Petri plates

Quantity: Each capsule contains 16.25 grams of medium sufficient for 250 ml media

Reaction: Reaction of 6.5% w/v aqueous solution at 25°C. pH: 5.6±0.2

pH: 5.40-5.80

Cultural Response

Cultural characteristics observed after an incubation at 20-25°C for 48 hours

Organism	Inoculum (CFU)	Growth	Recovery
Candida albicans ATCC 10231 (00054*)	50-100	luxuriant	>=70%
#Aspergillus brasiliensis ATCC 16404 (00053*)	50-100	luxuriant	>=70%
Candida albicans ATCC 2091 (00055*)	50-100	luxuriant	>=70%
Saccharomyces cerevisiae ATCC 9763 (00058*)	50-100	luxuriant	>=70%
Escherichia coli ATCC 25922 (00013*)	50-100	luxuriant	>=70%
Escherichia coli ATCC 8739 (00012*)	50-100	luxuriant	>=70%
Escherichia coli NCTC 9002	50-100	luxuriant	>=70%
Trichophyton rubrum ATCC 28191		luxuriant
Lactobacillus casei ATCC 334	50-100	luxuriant	>=70%

Key: (*) - Corresponding WDCM numbers. (#) - Formerly known as Aspergillus niger

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).

Product Information

More Information
Product Name	HiEncap™ Sabouraud Dextrose Agar
SKU	EC063CCL
Application/ Industry	Clinical, Food, Cosmetics
Product Type	HiEncap™
Physical Form	Granulated Media in Gelatin Capsule
Origin	Animal
Packaging type	HDPE Jar
References	1. Carlier G. I. M., 1948, Brit. J. Derm. Syph., 60:61. 2.Sabouraud K., 1892, Ann. Dermatol. Syphilol, 3:1061. 3.Bacteriological Analytical Manual, 8th Edition,
Customized Product Available	No