Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for cultivation of yeasts, moulds and aciduric microorganisms.
Composition
| Ingredients | g/L |
|---|---|
| Dextrose (Glucose) | 40.000 |
| Mycological, peptone | 10.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.6±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Sabouraud Dextrose Agar is a ready to use solid media in glass bottle. The medium is pre-sterilized, hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium in tubes as butts / slants or in plates as desired and allow to solidify. If on plate, either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically.
Principle And Interpretation
Sabouraud Dextrose Agar is Carlier's modification (1) of the formulation described by is a modification of Sabouraud Dextrose Agar which is described by Sabouraud (2) for the cultivation of fungi (yeasts, moulds), particularly useful for the fungi associated with skin infections. This medium is also employed to determine microbial contamination in food, cosmetics, and clinical specimens (3). Mycological Peptone provides nitrogenous compounds. Dextrose provides an energy source. High dextrose concentration and low pH favors fungal growth and inhibits contaminating bacteria from test samples (4).
Type of specimen
Clinical samples- skin scrapping, nail scrapping, etc.; Food & Dairy samples; Cosmetics
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6).
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines(7,8,9).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro Diagnostic use. For professional use only. Read the label before opening the pack. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet
- Further biochemical tests should be carried out for confirmation.
- It is recommended to store the plates at 24-30°C to avoid minimum condensation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile glass bottle containing clear to slightly opalscent Sabouraud Dextrose Agar
Colour of medium: Light yellow to amber coloured medium
Quantity of medium: 500ml of medium in glass bottle.
pH: 5.40-5.80
Sterility Check: Passes release criteria
Cultural Response: Cultural charactistics observed after melting the medium and pouring into sterile petri plates. The plates are inoculated with following test organisms and incubation at 20-25°C for 24-48 hours. Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar and fungus growth on Sabouraud Dextrose Agar.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Candida albicans ATCC 10231 (00054*) | 50-100 | Luxuriant (white colonies) | >=70% |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant | >=70% |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% |
| $Lactobacillus paracasei 334 | 50-100 | luxuriant | >=70% |
| Trichophyton rubrum ATCC 28191 | luxuriant |
Key: *Corresponding WDCM numbers. # Formerly known as Aspergillus niger . $ Formerly known as Lactobacillus casei.
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
References
- Carlier G. I. M., 1948, Brit. J. Derm. Syph., 60:61.
- Sabouraud K., 1892, Ann. Dermatol. Syphilol, 3:1061.
- Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. AOAC, Washington D.C.
- Murray PR, Baren EJ, Jorgensen JH, Pfaller MA, Yolken RH (editors) 2003, Manual of clinical Microbiology, 8th ed., ASM, Washington, D.C.
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C.,
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W.(2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
- American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington D.C.
- Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.
- Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C.
| Product Name | Sabouraud Dextrose Agar |
|---|---|
| SKU | SM063D |
| Customized Product Available | No |
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