Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Application
For cultivation of C.albicans in accordance with the harmonized method of USP/EP/BP/JP.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Mycological, peptone | 10.000 |
| Dextrose | 40.000 |
| Agar | 15.000 |
Formula adjusted, standardized to suit performance parameters.
Directions
Sabouraud Dextrose Agar is a ready to use solid media in glass bottle. The medium is pre-sterilized; hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium as desired and allowed to solidify.
Principle And Interpretation
Sabouraud Dextrose Agar is Carliers modification (1) of the formulation described by Sabouraud (2) for the cultivation of fungi (yeasts, moulds), particularly useful for the fungi associated with skin infections. This medium is also employed to determine microbial contamination in food, cosmetics, and clinical specimens (3).
Mycological peptone provides nitrogenous compounds. Dextrose provides an energy source. High dextrose concentration and low pH favours fungal growth and inhibits contaminating bacteria from test samples (4).
Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet. For heavily contaminated samples, the plate must be supplemented with inhibitory agents for inhibiting bacterial growth with lower pH.
Quality Control
Appearance: Sterile glass bottle containing clear to slightly opalescent Sabouraud Dextrose Agar.
Colour: Light Amber coloured medium
Quantity of medium: 100 ml of medium in glass bottle
Reaction: 5.40-5.80
Sterility test: Passes release criteria
Cultural response
Cultural characteristics after melting the medium and pouring into sterile petri plates. The plates are inoculated with following test organisms and incubation at 25°C for 7 days.
| Organism | Growth |
|---|---|
| Aspergillus niger ATCC 16404 | Luxuriant |
| Candida albicans ATCC 10231 | Luxuriant |
| Saccharomyces cerevisiae ATCC 9763 | Luxuriant |
| Trichophyton rubrum ATCC 28191 | Luxuriant |
Storage and Shelf Life
Store between 15-25°C. Use before expiry date on the label.
Reference
- Carlier G. I. M., 1948, Brit. J. Derm. Syph., 60:61.
- Sabouraud K., 1892, Ann. Dermatol. Syphilol, 3:1061.
- Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. AOAC, Washington D.C.
- Murray PR, Baren EJ, Jorgensen JH, Pfaller MA, Yolken RH (editors) 2003, Manual of clinical Microbiology, 8th ed., ASM, Washington, D.C.
| Product Name | Sabouraud Dextrose Agar |
|---|---|
| SKU | SMH063 |
| Customized Product Available | No |
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