Iron Medium Base is recommended for presumptive confirmation of Clostridium perfringens from from food in accordance with FDA BAM, 1998.iOn isolation of black colonies from suspected foods on TSC agar, the culture is enriched in Fluid thioglycollate medium. The enriched culture is tested for stormy fermentation in this medium with added whole milk ,
1.Downes, F.P. and Ito, K. 2001. Methods For The Microbiological Examination of Foods. APHA, Food 4 ed. Washington, D.C.2.FDA, U.S. 1998.Bacteriological Analytical Manual. 8 ed. Gaithersburg, MD: AOAC International.3.Abeyta, C Jr . and Wetherington, JH. 1994.J AOAC Int., 77(2): 351-6.