Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Shapton HiVeg Medium is used for enumeration of spores of Bacillus stearothermophilus which cause flat sour spoilage in canned foods with pH more than 4.5.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 5.0 |
| HiVeg hydrolysate | 2.5 |
| HiVeg extract | 3.0 |
| Yeast extract | 1.0 |
| Dextrose | 1.0 |
| Bromo cresol purple | 0.025 |
| Agar | 15.0 |
| Final pH (at 25°C) | 7.4 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Product Profile
Vegetable based (Code MV)
- MV645
- HiVeg peptone
- HiVeg hydrolysate
- HiVeg extract
Animal based (Code M)
- M645
- Peptic digest of animal tissue
- Casein enzymic hydrolysate
- Beef extract
Recommended for
Enumeration of spores of Bacillus stearothermophilus which cause flat sour spoilage in canned foods with pH more than 4.5.
Reconstitution
Reconstitution: 27.53 g/l
| Quantity on preparation (500g) | : 18.16 L |
| pH (25°C) | : 7.4 ± 0.2 |
| Supplement | : None |
| Sterilization | : 121°C / 15 minutes |
| Storage | : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Directions
Suspend 27.53 grams in 1000 ml distilled water. Soak for 5 minutes. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Shapton HiVeg Medium is prepared by using vegetable peptones in place of animal based peptones which makes the medium free of BSE/TSE risks. This medium is the modification of the medium formulated by Shapton and Hindes (1) for the enumeration of spores of Bacillus stearothermophilus which cause flat sour spoilage in canned foods with pH more than 4.5.
The sample under test is suspended in Ringer's salt solution and then added to sterile molten Shapton HiVeg Medium and is held at 100°C for 20 minutes. The temperature is then slightly raised to 108.4°C and maintained for 10 minutes after which it is cooled to 50°C and plates are poured. The plates are incubated at 55°C for 48 hours.
HiVeg peptone, HiVeg hydrolysate, HiVeg extract and yeast extract provides essential nitrogenous sources, vitamin B and other essential sources. Dextrose serves as fermentable carbohydrate source. Bromocrseol purple is the pH indicator used. On fermentaion and utilization of dextrose, the colour of the colony changes to yellow.
Quality Control
Appearance of powder
Greenish yellow coloured, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Light purple coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 2.75% w/v aqueous solution is pH 7.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 55°C for 18-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Colour of colony |
|---|---|---|---|
| Bacillus stearothermophilus (7953) | 102-103 | luxuriant | yellow |
References
- Shapton D.A. and Hindes W.R., 1963, Chemistry and Industry, p. 230.
| Product Name | Shapton HiVeg™ Medium |
|---|---|
| SKU | MV645 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Shapton D. A. and Hindes W. R., 1963, Chemistry and Industry, p. 230. |
| Customized Product Available | No |
Write Your Own Review
Dextrose Tryptone Agar
As low as
₹5,983.00
Thermophilic Acid Resistant Medium
As low as
₹6,101.00
Shapton Medium
As low as
₹6,101.00