Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Carbohydrate Consumption HiVeg Broth Base is recommended for cultivation and differentiation of Listeria species.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone No. 3 | 10.0 |
| Sodium chloride | 5.0 |
| HiVeg extract | 1.0 |
| Bromo cresol purple | 0.1 |
Final pH (at 25°C) 6.8 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Product Profile
Vegetable based (Code MV)
- MV1264
- HiVeg peptone No. 3
- HiVeg extract
Animal based (Code M)
- M1264
- Proteose peptone
- Beef extract
Specifications
- Recommended for
- Cultivation and differentiation of Listeria species.
- Reconstitution
- 16.1 g/l
- Quantity on preparation (500g)
- 31.05 L
- pH (25°C)
- 6.8 ± 0.2
- Supplement
- Carbohydrate solution
- Sterilization
- 121°C / 15 minutes.
- Storage
- Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 16.1 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Distribute into tubes with inverted Durhams tube. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Aseptically add 20 ml separately sterilized carbohydrate solution to give a final concentration of 0.5%. Mix well and dispense in sterile test tubes.
Principle and Interpretation
This medium is prepared by using HiVeg peptone No.3 and HiVeg extract in place of Proteose peptone and Beef extract respectively, which is free of BSE/TSE risks. Carbohydrate Consumption HiVeg Broth Base is the veg modification of Carbohydrate Consumption Broth Base employed for the cultivation and differentiation of Listeria species(1). Differentiation is based on fermentation of glucose, xylose, rhamnose, ribose, a -Methyl-D-mannoside and mannitol. HiVeg peptone and HiVeg extract provide carbon and nitrogen compounds including essential amino acids, vitamins and trace ingredients for bacterial metabolism.
On consumption of carbohydrate, medium turns acidic indicated by colour change of medium from purple to yellow. Bromo cresol purple is the pH indicator, which indicates acid production by turning yellow.
Quality Control
Appearance of powder
Beige coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity
Purple coloured, clear solution without any precipitate.
Reaction
Reaction of 1.61% w/v aqueous solution is pH 6.8 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18- 48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | # acid | w/ rhamnose (acid) | w/ rhamnose (gas) |
|---|---|---|---|---|---|
| Escherichia coli (25922) | 102-103 | luxuriant | - | + | + |
| Listeria monocytogenes (19111) | 102-103 | luxuriant | - | + | - |
| Listeria monocytogenes (19112) | 102-103 | luxuriant | - | + | - |
| Listeria monocytogenes (19117) | 102-103 | luxuriant | - | + | - |
| Staphylococcus aureus (25923) | 102-103 | luxuriant | - | - | - |
Key: # = without carbohydrate
| Product Name | Carbohydrate Consumption HiVeg™ Broth Base |
|---|---|
| SKU | MV1264 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Atlas R. M., 2004, Handbook of Microbiological Media, 3rd Edition, CRC Press, Washington D. C. |
| Customized Product Available | No |
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