Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
For rapid and direct identification of Listeria species in accordance with FDA BAM.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone, special | 30.000 |
| HM Extract # | 5.000 |
| Yeast Extract | 1.000 |
| Lithium Chloride | 9.000 |
| D-Xylose | 10.000 |
| Phenol Red | 0.120 |
| Chromogenic Mixture | 5.130 |
| Agar | 13.000 |
| CA Selective Supplement (FD181) | 1 vial |
| Ceftazidime | 2mg |
| Amphotericin B | 2.500mg |
| Final pH (at 25°C) | 7.3±0.1 |
Formula adjusted, standardized to suit performance parameters
Key: # - Equivalent to Meat extract
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
HiCrome™ Listeria Agar Base, Modified is a modification of the formulas by Notermans et al. (1) and Mengaud et al. (2) for the detection of Listeria species from food stuffs. This is also in accordance with the FDA BAM (3). This media helps in the presumptive identification of Listeria monocytogenes within 24-48 hours after pre-enrichment. The principle of detection is based on the specific chromogenic detection of β-glucosidase activity and also D-Xylose fermentation. Listeria species hydrolyse the purified chromogenic substrate in the medium giving blue coloured colonies. Since β-glucosidase activity is specific for Listeria species; other organisms cannot utilize the chromogenic substrate and therefore give white colonies. Differentiation between Listeria species is based on the property of D-Xylose fermentation. The colonies of L.ivanovii appear blue with a yellow halo (D-Xylose positive) while the colonies of L.monocytogenes and L.innocua appear blue without a yellow halo (D-Xylose negative). Peptone, yeast extract and HM extract provide nitrogenous substances, vitamin B complex and other essential growth nutrients. D-Xylose is the fermentable carbohydrate with phenol red as an indicator. Sodium chloride maintains the osmotic equilibrium. The added lithium chloride and CA Selective Supplement (FD181) inhibit growth of most gram-positive bacteria, gram-negative bacteria, yeasts and moulds.
Type of specimen
Food samples.
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile HiCrome™ Listeria Agar Plate in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles
Colour of medium
Red coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plates.
pH
7.10-7.50
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony | Xylose fermentation |
|---|---|---|---|---|---|
| Bacillus subtilis subsp. spizizenni ATCC 6633 (00003*) | >=104 | inhibited | 0% | ||
| Candida albicans ATCC 10231 (00054*) | >=104 | inhibited | 0% | ||
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | 0% | ||
| Listeria innocua ATCC 33090 (00017*) | 50-100 | luxuriant | >=50% | bluish green | negative reaction |
| Listeria ivanovii ATCC 19119 (00018*) | 50-100 | luxuriant | >=50% | bluish green | positive reaction, (yellow halo) |
| Listeria monocytogenes ATCC 19118 | 50-100 | luxuriant | >=50% | bluish green | negative reaction |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | >=104 | inhibited | 0% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | HiCrome™ Listeria Agar Plate |
|---|---|
| SKU | MP1417F |
| Customized Product Available | No |
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