• Home
  • MacConkey HiVeg™ Agar w/ CV, NaCl, 0.003% NR and 1.5% Agar
main product photo

MacConkey HiVeg™ Agar w/ CV, NaCl, 0.003% NR and 1.5% Agar

0 Review
As low as ₹1,044.00
Availability: In stock
Only %1 left
SKU:
MV081
For selective isolation and differentiation of coliform organisms and other enteric pathogens.

Intended Use

Recommended for selective isolation and differentiation of coliform organisms and other enteric pathogens.

Composition

Ingredients g/L
HiVeg® peptone No. 2 17.000
HiVeg® hydrolysate 1.500
HiVeg® peptone 1.500
Lactose 10.000
Synthetic detergent 1.500
Sodium chloride 5.000
Neutral red 0.030
Crystal violet 0.001
Agar 15.000
Final pH (at 25°C) 7.1±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 51.53 grams in 1000 ml purified/distilled water. Heat to boiling with gentle swirling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile Petri plates.

Principle And Interpretation

MacConkey agars are slightly selective and differential plating media mainly used for the detection and isolation of gram-negative organisms from dairy (1), food (2,3), water (4), pharmaceutical (5,6) and industrial sources (7). It is also recommended for the selection and recovery of the Enterobacteriaceae and related enteric gram-negative bacilli. USP recommends this medium for use in the performance of Microbial Limit Tests (6). MacConkey HiVeg® Agar is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associated with animal peptones.

These agar media are selective since the concentration of synthetic detergent, which inhibit gram-positive microbes, is low in comparison with other enteric plating media. The medium M081, which corresponds with, that recommended by APHA can be used for the direct plating of water samples for coliform bacilli, for the examination of food samples for food poisoning organisms (3) and for the isolation of Salmonella and Shigella species in cheese (1). Other than that this medium is also used for count of coli-aerogenes bacteria in cattle and sheep faeces (8), the count of coli-aerogenes and non-lactose fermenters in poultry carcasses (8), bacterial counts on irradiated canned minced chicken (9) and the recognition of coli-aerogenes bacteria during investigations on the genus Aeromonas (10,11).

MacConkey Agar is the earliest selective and differential medium for cultivation of enteric microorganisms from a variety of clinical specimens (12,13). The selective action of this medium is attributed to crystal violet and synthetic detergent, which are inhibitory to most species of gram-positive bacteria. Gram-negative bacteria usually grow well on the medium and are differentiated by their ability to ferment lactose. Lactose-fermenting strains grow as red or pink colonies and may be surrounded by a zone of acid precipitated bile. The red colour is due to production of acid from lactose, absorption of neutral red and a subsequent colour change of the dye when the pH of medium falls below 6.8. Lactose non-fermenting strains, such as Shigella and Salmonella are colourless, transparent and typically do not alter appearance of the medium. HiVeg® peptone No. 2, HiVeg® hydrolysate and HiVeg® peptone are sources of nitrogen, carbon, long chain amino acids and other nutrients. Lactose is a fermentable carbohydrate. Sodium chloride maintains the osmotic equilibrium. Synthetic detergent and crystal violet are selective agents that inhibit growth of gram-positive organisms. Neutral red is the pH indicator dye.

Type of specimen

Foodstuffs and dairy samples, water samples, pharmaceutical samples.

Specimen Collection and Handling

For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,5,3). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(4) For pharmaceutical samples, follow appropriate techniques for sample collection, processing as per guidelines.(5,6) After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions

Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations

  1. Though the medium is recommended for selective isolation, further biochemical and serological testing must be carried out for further confirmation.
  2. The surface of the medium should be dry when inoculated.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance
Light yellow to pink homogeneous free flowing powder

Gelling
Firm comparable with 1.5% Agar gel.

Colour and Clarity of prepared medium
Red with purplish tinge coloured clear to slightly opalescent gel forms in Petri plates.

Reaction
Reaction of 5.15% w/v aqueous solution at 25°C. pH : 7.1±0.2

pH
6.90-7.30

Cultural Response

Cultural response was observed after an incubation at 30-35°C for 18-72 hours. Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar.

Organism Inoculum (CFU) Growth Recovery Colour of colony
Corynebacterium diphtheriae type gravis >=104 inhibited 0%
Shigella flexneri ATCC 12022 (00126*) 50-100 fair to good 30-40% colourless
Salmonella Paratyphi A ATCC 9150 50-100 luxuriant >=50% colourless
Salmonella Abony NCTC 6017 (00029*) 50-100 luxuriant >=50% colourless
## Proteus hauseri ATCC 13315 50-100 luxuriant >=50% colourless
Salmonella Typhi ATCC 6539 50-100 luxuriant >=50% colourless
Staphylococcus epidermidis ATCC 12228 (00036*) >=104 inhibited 0%
Escherichia coli ATCC 8739 (00012*) 50-100 luxuriant >=50% pink-red with bile precipitate
Staphylococcus aureus subsp.aureus ATCC 6538 (00032*) >=104 inhibited 0%
Salmonella Paratyphi B ATCC 8759 50-100 luxuriant >=50% colourless
Escherichia coli ATCC 25922 (00013*) 50-100 luxuriant >=50% pink to red with bile precipitate
# Klebsiella aerogenes ATCC 13048 (00175*) 50-100 luxuriant >=50% pink to red
Salmonella Typhimurium ATCC 14028 (00031*) 50-100 luxuriant >=50% colourless
Enterococcus faecalis ATCC 29212 (00087*) 50-100 none - poor <=10% colourless to pale pink
Salmonella Enteritidis ATCC 13076 (00030*) 50-100 luxuriant >=50% colourless
Staphylococcus aureus subsp.aureus ATCC 25923 (00034*) >=104 inhibited 0%

Key :- * Corresponding WDCM
## Formerly known as Proteus vulgaris
# Formerly known as Enterobacter aerogenes

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (14,15).

Reference

  1. Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C.
  2. FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, D.C.
  3. Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.
  4. Lipps WC, Braun-Howland EB, Baxter TE, eds. Standard methods for the Examination of Water and Wastewater, 24th ed. Washington DC:APHA Press; 2023.
  5. The British Pharmacopoeia, 2022, Medicines and Healthcare products Regulatory Agency.
  6. The United States Pharmacopoeia-National Formulatory (USP-NF), 2022
  7. Williams, (Ed.), 2005, Official Methods of Analysis of the Association of Official Analytical Chemists, 19th Ed., AOAC, Washington, D.C
  8. Medrek T. F and Barnes Ella M., 1962, J. Appl. Bacteriol., 25(2),159-168
  9. Thornley Margaret J., 1957, J. Appl. Bacteriol., 20(2), 273-285.
  10. Eddy B. P., 1960, J. Appl. Bacteriol., 23(2).216-249.
  11. Barnes Ella M. and Shrimpton D. H., 1957, J. Appl. Bacteriol., 20(2),273-285.
  12. MacConkey A., 1905, J. Hyg., 5:333.
  13. MacConkey A., 1900, The Lancet, ii:20.
  14. Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition
  15. Murray P. R, Baron E, J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical Microbiology, ASM, Washington, D.C.
More Information
Product Name MacConkey HiVeg™ Agar w/ CV, NaCl, 0.003% NR and 1.5% Agar
SKU MV081
Product Type HiVeg™
Physical Form Powder
Origin Animal Free (Veg), Lactose
Packaging type HDPE
References 1.Murray P. R, Baron E, J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical Microbiology,ASM, Washington, D.C.
2.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C.
3.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C.
4.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, D.C.
5.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water andWastewater, 23rd ed., APHA, Washington, D.C.
6.The United States Pharmacopoeia, 2018, The United States Pharmacopeial Convention, Rockville, M.D.
7.Williams, (Ed.), 2005, Official Methods of Analysis of the Association of Official Analytical Chemists, 19th Ed., AOAC,Washington, D.C8.Medrek T. F and Barnes Ella M., 1962, J. Appl. Bacteriol., 25(2),159-1689.Barnes Ella M. and Shrimpton D. H., 1957, J. Appl. Bacteriol., 20(2),273-285.10.Thornley Margaret J., 1957, J. Appl. Bacteriol., 20(2), 273-285.
11.Eddy B. P., 1960, J. Appl. Bacteriol., 23(2).216-249.
12.MacConkey A., 1905, J. Hyg., 5:333.
13.MacConkey A., 1900, The Lancet, ii:20.
14.British Pharmacopoeia, 2016, The Stationery office British Pharmacopoeia.1
5.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition
Customized Product Available No
Write Your Own Review
You're reviewing:MacConkey HiVeg™ Agar w/ CV, NaCl, 0.003% NR and 1.5% Agar
Your Rating

Related Products

Upsell Products