Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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HiCombi™ Dual Performance Medium
Recommended for rapid growth of Enterobacteria, Pseudomonas, Staphylococci and Candida. Combination of solid (7 ml) and liquid (20 ml) media in single bottle.
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Biphasic HiCombi™ Salmonella Culture Systems#4fa25a
Intended use
Recommended for rapid growth of Enterobacteria, Pseudomonas, Staphylococci and Candida. Combination of solid (7 ml) and liquid (20 ml) media in single bottle.
Composition**
| Ingredients | g/L |
|---|---|
| Solid | |
| HM infusion powder # | 7.000 ml |
| BHI powder | 12.500 |
| Proteose peptone | 5.000 |
| Dextrose (Glucose) | 10.000 |
| Sodium chloride | 2.000 |
| Disodium phosphate | 5.000 |
| Agar | 2.500 |
| 15.000 | |
| Liquid | 20.000 ml |
Same as solid media without Agar
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Calf brain infusion from
Directions
Label the ready to use LQ033 bottle. Remove the top seal of the cap. Disinfect the part of the rubber stopper which is now exposed. Transfer the sample immediately into the culture bottle by puncturing the rubber stopper with the needle. Venting: Use sterile venting needle (LA038). Keep the bottle in an upright position preferably in a biological safety cabinet, place an alcohol swab over the rubber stopper and insert the venting needle with filter through it. Insertion and withdrawal of the needle should be done in a straight line. Discard the needle and mix the contents by gently inverting the bottle 2-3 times. Do not vent the bottle for anaerobic cultures. Incubate at 30-35°C for 18-24 hours and further for seven days. Recommended volume of blood to be tested in LQ033: 3-5 ml (For Pediatric use).
Principle And Interpretation
BHI Medium is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. It is also used to prepare the inocula for antimicrobial susceptibility testing. BHI Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth (1). BHI Broth is also the preferred medium for anaerobic bacteria, yeasts and moulds (2,3). This medium is nutritious and well buffered to support the growth of wide variety of organisms (3,4,5).
Proteose peptone, HM infusion powder and BHI powder serve as sources of carbon, nitrogen, essential growth factors, amino acids and vitamins. Dextrose serves as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium whereas sodium chloride maintains the osmotic equilibrium of the medium.
Type of specimen
Clinical samples: Blood
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (8,9).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical and serological testing is required for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: In a sterile glass bottle combination of broth and one agar coated surface.
Colour of Agar medium: Yellow coloured medium
Colour of liquid medium: Amber coloured solution
Quantity of medium: 7ml of solid medium in glass bottle 20ml of liquid medium in glass bottle
pH of Agar medium: 7.20-7.60
pH of liquid medium: 7.20-7.60
Sterility Check: Passes release criteria
Cultural response: Cultural characteristics was observed after incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth in liquid medium |
Growth on agar medium |
|---|---|---|---|
| Candida albicans ATCC 10231 (00054*) | 50-100 | Luxuriant | Luxuriant |
| Haemophilus influenzae ATCC 19418 | 50-100 | Luxuriant | Luxuriant |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | Luxuriant | Luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | Luxuriant | Luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | Luxuriant | Luxuriant |
| Neisseria meningitidis ATCC 13090 | 50-100 | Luxuriant | Luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | Luxuriant | Luxuriant |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | HiCombi™ Dual Performance Medium |
|---|---|
| SKU | LQ033 |
| Customized Product Available | No |
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