Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for cultivation of anaerobic microorganisms, including very fastidious organisms from clinical specimens.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 15.000 |
| Soya peptone | 5.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 5.000 |
| L-Cysteine | 0.500 |
| Hemin | 0.005 |
| Agar | 13.500 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 44.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Add the rehydrated contents of one vial of Vitamin K1 supplement (FD114). Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 5% v/v sterile defibrinated sheep blood. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Anaerobic Blood Agar base serves as a nutritious, nonselective medium allowing the cultivation of not only fastidious anaerobes but also of aerobic and microaerophillic microorganisms (1). It promotes both typical pigment formation in Bacteroides melaningenicus and displays double haemolytic reaction in Clostridium perfringens with added blood to the medium base. The inner zone of haemolysis is due to toxin and the outer zone of incomplete haemolysis to toxin (lecithinase activity). Tryptone, soya peptone and yeast extract in the medium provides carbon and nitrogenous source, long chain amino acids, vitamins and other essential nutrients. Presence of Hemin and Vitamin K1 supports the growth of typical fastidious bacteria like Bacteroides species and gram positive spore bearers like Clostridium species. Addition of blood provides nutrients and helps to differentiate haemolytic organisms. Sodium chloride helps in maintaining the osmotic equilibrium.
Type of specimen
Clinical samples- stool, abscess
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (2,3).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Due to nutritional variations, certain strains may show poor growth.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within expiry period when stored at the recommended temperature.
Quality Control
Appearance
Yellow to tan coloured homogeneous free flowing powder
Colour and Clarity of prepared medium
Basal medium: Yellow coloured; with addition of 5% v/v sterile, defibrinated sheep blood: cherry red coloured
Basal medium: slightly opalescent; After addition of 5% v/v sterile, defibrinated sheep blood: opaque gel in petri plates
Reaction
Reaction of 4.4% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after 24-48 hours at 35-37°C with 5-10% CO2
| Organism | Growth |
|---|---|
| Bacteroides fragilis ATCC 25285 | luxuriant |
| Bacteroides melaninogenicus ATCC 25611 | luxuriant |
| Peptostreptococcus anaerobius ATCC 27337 | luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
Reference
- Dowell, Jr., V.R., Lombard, G.L, Thompson, F.S, Armfield, A.Y.: Media for isolation, characterization and identification of obligately anaerobic bacteria- US Department of Health and Human services, centers for Disease Control (1977).
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
| Product Name | Anaerobic Blood Agar Base |
|---|---|
| SKU | M975A |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Ljungdahl L. G., Adams M. W., Barton L. L., Ferry J. G., Johnson M. K ., Biochemistry and Physiology of AnaerobicBacteria. Microbiology.Springer publication |
| Customized Product Available | No |
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