Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended for isolation and cultivation of Group A and Group B Streptococci from throat cultures and other clinical samples.
Composition**
| Ingredients | g/L |
|---|---|
| Trptone | 14.500 |
| Soya peptone | 5.000 |
| Sodium chloride | 5.000 |
| Growth Factors | 1.500 |
| Agar | 14.000 |
| Neo Selective Supplement(FD149) | 1 vial |
| Neomycin | 30.000mg |
| Sterile defibrinated sheep blood | 50 ml |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Group B streptococcus (GBS) infection is a common bacterial infection that is rarely serious in adults, but can be life-threatening to newborns. Group A Streptococci commonly causes strep throat and rarely, a potentially deadly destruction of flesh. Anaerobic Blood Agar Base with Neomycin Supplement is used for the isolation of Group A and Group B Streptococci from clinical specimens (1). This medium was originally formulated by Blanchette and Lawrence (2), by addition of the antibiotic Neomycin to sheep blood agar. This addition improved the detection of Group A & B Streptococci, while inhibiting the growth of the other accompanying haemolytic organisms.
Tryptone and soya peptone in the medium provide carbon and nitrogenous compounds, long chain amino acids, vitamins and other essential growth nutrients. Growth factors and defibrinated sheep blood together supply enrichment for growth of fastidious organisms. Sodium chloride helps in maintaining the osmotic equilibrium. Neo Selective Supplement (FD149) helps to suppress the normal flora thereby enhancing recovery of Group A and Group B Streptococci.
Type of specimen
Clinical samples- Throat swabs, Vaginal or rectal secretions
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precaution:
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Anaerobic Blood Agar Plate w/ Neomycin in 90mm disposable plate with smooth surface and absence of black particles/ cracks/bubbles
Colour of medium
Cherry red coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plate
pH
7.10-7.50
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed in presence of 5-10% CO2 after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Haemolysis |
|---|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | none-poor | <=10% | none |
| Staphylococcus aureus subsp.aureus ATCC 25923 (00034*) | 50-100 | none-poor | <=10% | none |
| Streptococcus agalactiae ATCC 13813 | 50-100 | good-luxuriant | >=50% | beta |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant | >=50% | beta |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Anaerobic Blood Agar Plate w/ Neomycin |
|---|---|
| SKU | MP1345 |
| Customized Product Available | No |
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