Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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V-8 Medium for Lactobacilli is recommended for cultivation and enumeration of lactobacilli.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| V-8 vegetable juice | 10.000 |
| Tryptose | 10.000 |
| Beef extract | 3.000 |
| Lactose | 5.000 |
| Bromo cresol green | 0.100 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.7±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 43.1 grams in 1000 ml warm distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Principle And Interpretation
The genus Lactobacillus is by far the largest of the genera included in Lactic Acid Bacilli (LAB). Lactic acid bacilli are widely distributed in nature and are generally harmless to humans. However, Lactobacillus plantarum and Lactobacillus casei have been implicated in pathological conditions (1).
V-8 Medium for Lactobacilli is recommended by APHA (2) for cultivation and enumeration of lactobacilli.
The medium contains tryptose and lactose, which are the energy sources. Beef extract provides carbon, nitrogen, vitamins and minerals necessary to support yeast growth. V-8 vegetable juice is a blend of 8 vegetable juices that supplies trace ingredients to stimulate growth. Bromocresol green acts as pH indicator for acid production.
Quality Control
Appearance
Cream to blue homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity of prepared medium
Green to bluish green coloured clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 4.31% w/v aqueous solution at 25°C. pH : 5.7±0.2
pH
5.50-5.90
Cultural Response
M954: Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Lactobacillus acidophilus ATCC 4356 | 50-100 | good-luxuriant | >=50% | yellow |
| Lactobacillus casei ATCC 9595 | 50-100 | good-luxuriant | >=50% | yellow |
| Lactobacillus plantarum ATCC 8014 | 50-100 | good-luxuriant | >=50% | yellow |
Storage and Shelf Life
Store dehydrated powder and prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
- Salminen Seppo, von Wright A. and Ouwehand A. C., Lactic Acid Bacteria: Microbiological and Functional Aspects, 3rd Edi, Marcel and Dekker. NY.Basel.
- Speck M. L., (Eds.), 1984, Compendium of Methods for the Microbiological Examination of Foods, 2nd ed., APHA, Washington, D.C.
| Product Name | V-8 Medium for Lactobacilli |
|---|---|
| SKU | M954 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Salminen Seppo, von Wright A. and Ouwehand A. C., Lactic Acid Bacteria: Microbiological and Functional Aspects, 3rdEdi, Marcel and Dekker. NY.Basel. |
| Customized Product Available | No |
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