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Potato Dextrose Agar w/ Agar

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SKU:

M937

For cultivation of yeasts and moulds from dairy and other food products.

Description

Composition

Ingredients	Gms / Litre
Potatoes, infusion from	200.000
Dextrose	20.000
Agar	30.000
Final pH (at 25°C)	5.6±0.2

Formula adjusted, standardized to suit performance parameters

Directions

Suspend 54 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before dispensing. In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml. of sterile, cooled medium is approximately 1 ml. Do not heat the medium after addition of the acid.

Principle And Interpretation

Potato Dextrose Agar is recommended by APHA (1) and F.D.A (2) for plate counts of yeasts and moulds in the examination of foods and dairy products (3). Incorporation of 3% agar enhances sporulation in the medium.

Potato infusion and dextrose promote luxuriant fungal growth. Adjusting the pH of the medium by tartaric acid to 3.5, inhibits the bacterial growth. Heating the medium after acidification should be avoided as it may hydrolyse the agar which can render the agar unable to solidify.

Quality Control

Appearance: Cream to yellow homogeneous free flowing powder

Gelling: Firm, comparable with 3.0% Agar gel

Colour and Clarity of prepared medium: Light amber coloured clear to slightly opalescent gel forms in Petri plates.

Reaction: Reaction of 5.4% w/v aqueous solution at 25°C. pH : 5.6±0.2

pH: 5.40-5.80

Cultural Response

M937: Cultural characteristics observed after an incubation at 22-25°C for 4-5 days.

Organism	Inoculum (CFU)	Growth	Ascospore formation	Recovery
*Aspergillus brasiliensis ATCC 16404	50-100	luxuriant	negative
Candida albicans ATCC 10231	50-100	luxuriant	negative	>=70%
Saccharomyces cerevisiae ATCC 9763	50-100	luxuriant	positive	>=70%

Key: * - Formerly known as Aspergillus niger

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

Reference

Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., APHA, Washington, D.C.
FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.
Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C.

Product Information

More Information
Product Name	Potato Dextrose Agar w/ Agar
SKU	M937
Product Type	Regular
Physical Form	Powder
Origin	Animal Free (Veg)
Packaging type	HDPE
References	1. Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C.2.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C.
Customized Product Available	No