Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use:
Recommended for the detection, isolation and enumeration of yeasts and moulds from clinical and non clinical samples.
Composition**
| Ingredients | g/L |
|---|---|
| Malt extract | 30.000 |
| Mycological peptone | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate
Principle And Interpretation
The laboratory diagnosis of fungal infection relies largely on direct as opposed to indirect methods. The use of malt and malt extracts for the propagation of yeasts and moulds is quite common. Reddish (1) described a culture medium prepared from malt extract that was a satisfactory substitute for wort. Malt Extract Medium is similar to the formula of Galloway and Burgess (2) used for the detection, isolation and enumeration of yeasts and moulds.
Malt extract provides an acidic environment and nutrients favorable for growth and metabolism of yeasts and moulds. Mycological peptone rapidly gives a luxuriant growth with typical morphology and pigmentation. For mycological count, it is advisable to adjust the reaction of medium more acidic with addition of 10% lactic acid. Antibiotics may be added as sterile solutions to the molten medium immediately before pouring into sterile Petri plates (3) in order to suppress bacterial growth.
Type of specimen
Clinical samples : skin scrappings, nail scrappings, etc.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical tests must be carried out for further identification
- It is recommended to store the plates at 24-30°C to avoid condensation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Malt Extract Agar in 90 mm disposable plate with smooth surface and absence of black particles/cracks/bubbles
Colour of medium
Light amber coloured medium.
Quantity of Medium
25ml of medium in 90 mm plate.
pH
5.20-5.60
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 48-72 hours. Incubate for 7 days for Trichophyton species.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | >=70% |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | luxuriant | >=70% |
| $Penicillium rubens ATCC 9179 | 50-100 | luxuriant | - |
| ^Trichophyton interdigitale ATCC 9533 | 50-100 | luxuriant |
Key: (*) Corresponding WDCM numbers.
# Formerly known as Aspergillus niger
^Formerly known as Trichophyton mentagrophytes
$ Formerly known as Penicillium chrysogenum
Storage and Shelf Life
On receipt, store between 20-30°C. Use before expiry date on the label. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Malt extract Agar Plate |
|---|---|
| SKU | MP137 |
| Customized Product Available | No |
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