Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use:
Recommended for cultivation of Mycobacterium tuberculosis.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| AcicaseTM | 10.000 |
| HM peptone B # | 3.000 |
| L-Asparagine | 3.000 |
| Potato starch | 15.000 |
| Ferric ammonium citrate | 0.100 |
| Magnesium sulphate | 0.015 |
| Dipotassium hydrogen phosphate | 3.500 |
| Citric acid | 0.100 |
| Agar | 15.000 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
$ Equivalent to Casein acid hydrolysate
Directions
Suspend 49.72 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the media completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to around 55°C and aseptically add egg yolk emulsion (prepared from 10 sterile egg yolks and 25 ml sterile saline, to which 1 ml of sterile 20% dextrose solution and 13 ml of 1% malachite green solution is added) and add 40 ml sterile glycerol. Mix thoroughly and dispense in tubes, then allow it to solidify as slants.
Principle And Interpretation
Peizer TB Medium was formulated by Peizer et al (1) for the cultivation of Mycobacterium tuberculosis and also for the diagnosis of tuberculosis. It can also be used for determining the sensitivity of Mycobacterium tuberculosis to therapeutic agents (2).
HM peptone B and AcicaseTM are the rich sources of nitrogen and some additional growth factors for the growth of tubercle bacilli (3). Egg yolk emulsion provides fatty acids and proteins required for the metabolism of Mycobacteria. L-Asparagine and starch serves as the amino acid and carbohydrate source respectively. Citric acid holds certain inorganic cations in solution. Malachite green inhibits certain contaminating bacteria.
Type of specimen
Clinical samples - Sputum sample
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to light tan homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light green coloured opaque gel forms in tube as slants
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 2-4 weeks.
| Organism | Growth |
|---|---|
| Mycobacterium fortuitum ATCC 6841 | luxuriant |
| Mycobacterium kansasii ATCC 12478 | luxuriant |
| M. tuberculosis H37RV ATCC 25618 | luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Peizer TB Medium Base |
|---|---|
| SKU | M867 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Peizer and Schecter, 1950, Am. J. Clin. Path., 20:682.2.Peizer, Widelock and Schecter, 1951, Am. J. Clin. Path., 21:982.3.Dubos and Middlebrook, 1947, Am. Rev. Tuberc., 56:334. |
| Customized Product Available | No |
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