Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
For cultivation of Mycobacterium tuberculosis.
Composition
| Ingredients | g / L |
|---|---|
| L-Asparagine | 3.60 |
| Potassium dihydrogen phosphate | 2.40 |
| Magnesium sulphate | 0.24 |
| Magnesium citrate | 0.60 |
| Potato flour | 30.00 |
| Malachite green | 0.40 |
| Whole egg emulsion | 1000.00 ml |
| Glycerol | 12.00 ml |
Formula adjusted, standardized to suit performance parameters
Directions
Inoculate either the sputum sample previously subjected to decontamination and concentration process or the pure culture of Mycobacteria isolated from a clinical sample on the surface of the slants. Incubate the slants at 35-37°C and examine the slants every week upto 8 weeks.
Principle And Interpretation
L.J. Medium is prepared as per the Jensen's (1) modification of the original formulation of Lowenstein (2). The egg based medium supports the growth of a wide variety of Mycobacteria and can also be used for niacin testing (3). Glycerol provides fatty acids. Malachite green serves as an inhibitor as well as pH indicator. Formation of blue zones indicates a decrease in pH by Gram-positive contaminants (e.g. Streptoccocci) and yellow zones of dye destruction by Gram-negative bacilli. Proteolytic contaminants cause localised or complete digestion of the medium (4).
Type of specimen
Clinical samples : Sputum
Specimen Collection and Handling
For clinical samples:Refer appropriate references for standard test procedures of decontamination and isolation (5,6). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Certain gram-positive contaminants (e.g. Streptococci) and gram-negative bacilli may grow on the medium.
- Certain Saprophytes may also grow on the medium.
- Proteolytic contaminants cause localized or complete digestion of medium.
- Further biochemical and serological identification is necessary for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Pale bluish green coloured, opaque smooth slant.
Cultural response
Cultural characteristics within 2-4 weeks at 35-37°C, with 5-10% CO2 (further growth may be observed for 8 weeks).
| Organism | Growth | Colony characteristics | Inoculum |
|---|---|---|---|
| Mycobacterium bovis ATCC 25523 | Luxuriant | luxuriant, tiny, transparent, white colonies | |
| M. tuberculosis H37Rv ATCC 25618 | Luxuriant | luxuriant, granular, rough, dry warty, friable colonies. | Standardized inoculum giving approximately 1000000 cfu/ml |
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Acid Egg Medium Slant |
|---|---|
| SKU | SL008 |
| Customized Product Available | No |
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