Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Dextrose Peptone Broth
Used for the cultivation of fastidious organisms,enumeration of thermophilic bacteria from canned food and for routine sterility testing.
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Intended Use
Recommended for the cultivation of fastidious organisms, enumeration of thermophilic bacteria from canned food and for routine sterility testing.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 20.000 |
| Dextrose (Glucose) | 10.000 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 35 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C.
Principle And Interpretation
Dextrose Peptone Broth is formulated as per the procedures described by Williams (5) for the cultivation of microorganisms that are fastidious, or present in small numbers and also for the enumeration of thermophilic bacteria responsible for flat-sour spoilage of canned foods. This medium is recommended by AOAC for the routine cultivation purposes (1). Dextrose is the readily available energy source for most of the organisms. Dextrose Peptone Broth can also be used for routine sterility testing. Supplementation of the medium with blood provides additional nutrients.
Type of specimen
Food samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to light yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured, clear to slightly opalescent solution in tubes
Reaction
Reaction of 3.5% w/v aqueous solution at 25°C. pH: 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use.
Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Dextrose Peptone Broth |
|---|---|
| SKU | M650 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Association of Official, Analytical, Chemists, 1978, Bacteriological Analytical Manual, 5th Ed, AOAC, Washington, D.C. 2.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition. 3.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manualof Clinical Microbiology, 11th Edition. Vol. 1. 4.Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed.,American Public Health Association, Washington, D.C. 5.Williams O.B., 1936, Food Res., 1(3):217. |
| Customized Product Available | No |
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