Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for general cultivation of organisms.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 20.000 |
| Dextrose (Glucose) | 10.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.2±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 50 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50° C. mIx well and pour into sterie Petri plates.
Principle And Interpretation
Dextrose Peptone Agar is formulated as suggested by Williams (8) for the cultivation of microorganisms, which are fastidious, or present in small numbers, and also for the enumeration of the thermophilic bacteria responsible for flat sour spoilage of canned foods. This medium is recommended by AOAC for the routine cultivation purpose (2).
Peptone supplies amino acids, peptides etc. for the growth of the organisms. Dextrose is the readily available energy source for the most of the organisms. The agar medium is also used as an excellent basal agar for the Glucose Blood Agar preparation. In the special Petri plates, it can support good growth of the anaerobic microorganisms.
Type of specimen
Food and dairy samples; Water samples.
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,6,7). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(3) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light yellow coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 5.0% w/v aqueous solution at 25°C. pH : 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | >=70% |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant | >=70% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Dextrose Peptone Agar |
|---|---|
| SKU | M649 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed.,Washington D.C. 2.Association of Official Analytical Chemists, 1978, Bacteriological Analytical Manual, 5th ed., AOAC, Washington, D.C. 3.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater,23rd ed., APHA, Washington, D.C. 4.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition. 5.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual ofClinical Microbiology, 11th Edition. Vol. 1. 6.Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th EdAmerican Public Health Association, Washington, D.C. 7.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C. 8.Williams O.B., 1936, Food Res., 1(3):217. |
| Customized Product Available | No |
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