Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Minerals Modified Glutamate Agar Base (Twin Pack)
Recommended for the enumeration of Escherichia coli from meat and meat products. The composition and performance criteria of this medium are as per the specifications laid down in ISO 6391:1997.
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Intended Use
Recommended for the enumeration of Escherichia coli from meat and meat products. The composition and performance criteria of this medium are as per the specifications laid down in ISO 6391:1997, ISO 11866-1:2005(E) IDF 170-1:2005(E) and IS 15463: 2004.
Composition
ISO 6391:1997 ISO 11866-1:2005(E) IDF 170-1:2005(E) and IS 15463: 2004. Specification-Mineral Modified Glutamate Agar
| Ingredients | g/L |
|---|---|
| Sodium glutamate | 6,35 g |
| Lactose | 10,0 g |
| Sodium formate | 0,25 g |
| L(-)-cystine | 0,02 g |
| L(-)-aspartic acid | 0,024 g |
| L(+)-arginine | 0,02 g |
| Thiamine | 0,001 g |
| Nicotinic acid | 0,001 g |
| Pantothenic acid | 0,001 g |
| Magnesium sulfate (MgSO4.7H2O) | 0,100 g |
| Ammonium iron(II) citrate | 0,010 g |
| Calcium chloride (CaCl2.2H2O) | 0,010 g |
| Dipotassium hydrogen phosphate | 0,90 g |
| Ammonium chloride | 2,5 g |
| Agar | 12 g to 18g |
| Final pH (at 25°C) | 6.7±0.2 |
M6431- Mineral Modified Glutamate Agar
| Ingredients | g/L |
|---|---|
| Part A | |
| Lactose | 10.000 |
| Dipotassium hydrogen phosphate | 0.900 |
| Sodium formate | 0.250 |
| L-Cystine | 0.020 |
| L-Aspartic acid | 0.024 |
| L-Arginine | 0.020 |
| Thiamine | 0.001 |
| Nicotinic acid (Niacin) | 0.001 |
| Pantothenic acid | 0.001 |
| Ferric ammonium citrate | 0.010 |
| Calcium chloride dihydrate | 0.010 |
| Magnesium sulphate heptahydrate | 0.100 |
| Agar | 15.000 |
| Part B | |
| Sodium glutamate | 6.350 |
| Final pH (at 25°C) | 6.7±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 26.29 grams (the equivalent weight of dehydrated medium per litre)of Part A and 6.35 grams of Part B in 1000 ml purified / distilled water containing 2.5 grams ammonium chloride. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 10lbs (115°C) for 10 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Folpmers (1) described a glutamic acid based chemically defined medium for the enumeration of coliform bacteria from water and wastewater. This glutamate-containing medium was later modified by Gray (2), by the addition of lactose, which gave less false positive results when compared to MacConkey Broth.
Mineral Modified Glutamate Agar Base is recommended for the enumeration of Escherichia coli from meat and meat products by colony count at 44°C by membrane filtration technique(3). Anderson and Baird-Parker (4) described a direct plate method for the rapid enumeration of E. coli in foods and this method was modified by a resuscitation procedure using Mineral Modified Glutamate Agar with successful recovery of damaged cells from frozen, dried, heat processed or low pH foods. Mineral Modified Glutamate Agar Base (Twin Pack)is also recommended by ISO Committee(5)
This media contain a variety of nutrients including salts, amino acids and vitamins. Lactose is the fermentable carbohydrate. Because of the nutrients, this media is superior for enumerating coliforms in water and wastewater as it satisfies most of the nutritional requirements of coliforms.
Type of specimen
Food samples: meat and meat products
Specimen Collection and Handling:
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (6). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets
Limitations :
- Due to variable nutritional requirements, some strains show poor growth on this medium.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Part A: Off-white to light yellow homogeneous free flowing powder Part B: Colourless to white needles
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of the medium (2.634% w/v Part A + 0.635% w/v Part B + 0.25% ammonium chloride) is at 25°C. pH: 6.7±0.2
pH
6.50-6.90
Cultural Response
Cultural characteristics observed after an incubation at 44°C for 24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=50% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Minerals Modified Glutamate Agar Base (Twin Pack) |
|---|---|
| SKU | M643I |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Folpmers T., 1948, Ant. V. Leeuwenhoek, J. Microbiol. Serol., 14:58. 2.Gray R.D., 1959, J. Hyg. Camb., 57:249. 3.Anderson J. M., and Baird Parker A. C., 1975, J. Appl. Bacteriol., 39:1 4.International Organization for Standardization (ISO), 1988, Draft, ISO/DIS 6391. |
| Customized Product Available | No |
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