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Tryptone Bile Glucuronic Agar

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M1591

Selective agar for the detection and enumeration of Escherichia coli in foodtuffs, animal feed, water and clinical samples.

Description

Tryptone Bile Glucuronic Agar is selective agar for the detection and enumeration of Escherichia coli in foodstuffs and animal feed and water. It differentiates most E.coli species from other coliforms. E.coli absorbs the chromogenic substrate 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide.The enzyme ß-glucorinodase splits the bond between the chromophere 5-bromo-4-chloro-3-indolyl and the ß-D-glucoronide. E.coli colonies appear as blue green coloured colonies.

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Product Name	Tryptone Bile Glucuronic Agar
SKU	M1591
Product Type	Regular
Physical Form	Powder
Origin	Animal
Packaging type	HDPE
References	1.Frampton E W, Restaino L, Blaszko L.1988.Eavaluation of ß-glucoridase substrate 5-bromo-4-chloro3-indolyl-B-D-glucuonide (X-GLUC) in a 24 hour direct plating method for Escherichia coli. J. Food Protection 51:402-404.2.Killian M. and Bolow P 1976 Rapid diagnosis of Enetrobacteriacea I. Detection of bacterial glycosidases. Acta Rattol.Microbiol Scand Sct B 84245:251.3.Ley A N, Bowers R J, Wolfe S 1988 Indocyl –B-D-glcuaoride, a novel chromogenic coli reagent for the detection andenumeration of Escherichia coli in environmental samples. Canadian Journal of Microbiology 34:690-693.4.International Standard ISO 16649-2: 1999.Microbiology of food and animal feeding stuffs- Horizontal method for theenumeration of presumptive Escherichia coli; Part 2: Colony-count technique at 44˚C using 5-bromo-4-chloro-3-indolyl-ß-D-glucoronic acid.5.Baird R.B ., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater, 23rd ed., APHA, Washington, D.C.6.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.7.Jorgensen,J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.8.Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C
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