Nutrient Agar No.2 is used as a general purpose culture medium, also can be used for the microbiological analysis of water as per Czech Standards. It can also be used for sterility testing of aerobes and also for maintenance of subcultures.HM peptone B and peptone provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients to the bacteria. Sodium chloride maintains osmotic equilibrium of the medium.
1. Clesceri L. S, Greenberg A. E. and Eaton A. D., (Eds.), 1998, Standard Methods for the Examination of Water andWastewater, 20th Ed., APHA, Washington, D.C.2.American Public Health Association, 1978, Standard Methods for the Examination of Dairy Products, 14th Ed., APHA,Inc., Washington, D.C.3.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williamsand Wilkins, Baltimore.